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1v5d

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{{STRUCTURE_1v5d| PDB=1v5d | SCENE= }}
{{STRUCTURE_1v5d| PDB=1v5d | SCENE= }}
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'''The crystal structure of the active form chitosanase from Bacillus sp. K17 at pH6.4'''
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===The crystal structure of the active form chitosanase from Bacillus sp. K17 at pH6.4===
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==Overview==
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Crystal structures of chitosanase from Bacillus sp. K17 (ChoK) have been determined at 1.5 A resolution in the active form and at 2.0 A resolution in the inactive form. This enzyme belongs to the family GH-8, out of 93 glycoside hydrolase families, and exhibits the substrate specificity of subclass II chitosanase. The catalytic site is constructed on the scaffold of a double-alpha(6)/alpha(6)-barrel, which is formed by six repeating helix-loop-helix motifs. This structure is quite different from those of the GH-46 chitosanases and of GH-5. Structural comparison with CelA (a cellulase belonging to the same family GH-8) suggests that the proton donor Glu122 is conserved, but the proton acceptor is the inserted Glu309 residue, and that the corresponding Asp278 residue in CelA is inactivated in ChoK. The four acidic residues, Asp179, Glu309, Asp183 and Glu107, can be involved in substrate recognition through interactions with the amino groups of the glucosamine residues bound in the -3, -2, -1 and +1 sites, respectively. The hydrophobic Trp235, Trp166, Phe413 and Tyr318 residues are highly conserved for binding of the hexose rings at the -3, -2, +1 and +2 sites, respectively. These structural features indicate that enzymes in GH-8 can be further divided into three subfamilies. Different types of chitosanases are discussed in terms of convergent evolution from different structural ancestors.
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(as it appears on PubMed at http://www.pubmed.gov), where 15465062 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15465062}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Crystal structure of family GH-8 chitosanase with subclass II specificity from Bacillus sp. K17., Adachi W, Sakihama Y, Shimizu S, Sunami T, Fukazawa T, Suzuki M, Yatsunami R, Nakamura S, Takenaka A, J Mol Biol. 2004 Oct 22;343(3):785-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15465062 15465062]
Crystal structure of family GH-8 chitosanase with subclass II specificity from Bacillus sp. K17., Adachi W, Sakihama Y, Shimizu S, Sunami T, Fukazawa T, Suzuki M, Yatsunami R, Nakamura S, Takenaka A, J Mol Biol. 2004 Oct 22;343(3):785-95. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15465062 15465062]
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Crystallization and preliminary X-ray analyses of the active and the inactive forms of family GH-8 chitosanase with subclass II specificity from Bacillus sp. strain K17., Sakihama Y, Adachi W, Shimizu S, Sunami T, Fukazawa T, Suzuki M, Yatsunami R, Nakamura S, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2081-3. Epub 2004, Oct 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15502334 15502334]
[[Category: Chitosanase]]
[[Category: Chitosanase]]
[[Category: Adachi, W.]]
[[Category: Adachi, W.]]
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[[Category: Glycosil hydrolase]]
[[Category: Glycosil hydrolase]]
[[Category: Hydrolase]]
[[Category: Hydrolase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:05:56 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:57:09 2008''

Revision as of 11:57, 29 July 2008

Image:1v5d.png

Template:STRUCTURE 1v5d

The crystal structure of the active form chitosanase from Bacillus sp. K17 at pH6.4

Template:ABSTRACT PUBMED 15465062

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of family GH-8 chitosanase with subclass II specificity from Bacillus sp. K17., Adachi W, Sakihama Y, Shimizu S, Sunami T, Fukazawa T, Suzuki M, Yatsunami R, Nakamura S, Takenaka A, J Mol Biol. 2004 Oct 22;343(3):785-95. PMID:15465062

Crystallization and preliminary X-ray analyses of the active and the inactive forms of family GH-8 chitosanase with subclass II specificity from Bacillus sp. strain K17., Sakihama Y, Adachi W, Shimizu S, Sunami T, Fukazawa T, Suzuki M, Yatsunami R, Nakamura S, Takenaka A, Acta Crystallogr D Biol Crystallogr. 2004 Nov;60(Pt 11):2081-3. Epub 2004, Oct 20. PMID:15502334

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