9nh0

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m (Protected "9nh0" [edit=sysop:move=sysop])
Current revision (05:40, 17 September 2025) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 9nh0 is ON HOLD
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==In situ cryo-EM structure of PR and DotA-IcmX of the Legionella Dot/Icm T4SS machine at C1 symmetry==
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<StructureSection load='9nh0' size='340' side='right'caption='[[9nh0]], [[Resolution|resolution]] 4.63&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[9nh0]] is a 141 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_pneumophila_subsp._pneumophila Legionella pneumophila subsp. pneumophila]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9NH0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9NH0 FirstGlance]. <br>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.63&#8491;</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9nh0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9nh0 OCA], [https://pdbe.org/9nh0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9nh0 RCSB], [https://www.ebi.ac.uk/pdbsum/9nh0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9nh0 ProSAT]</span></td></tr>
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</table>
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== Function ==
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[https://www.uniprot.org/uniprot/Q5ZYC0_LEGPH Q5ZYC0_LEGPH]
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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The Dot/Icm machine in Legionella pneumophila is one of the most versatile type IV secretion systems (T4SSs), with a remarkable capacity to translocate over 330 different effector proteins across the bacterial envelope into host cells. At least 27 Dot and Icm proteins are required for assembly and function of the system, yet the architecture and activation mechanism remain poorly understood at the molecular level. Here, we deploy cryo-electron microscopy to reveal in-situ structures of the Dot/Icm machine at near-atomic resolution. Importantly, two proteins essential for effector translocation, DotA and IcmX, form a pentameric protochannel at an inactive state. Upon activation, the DotA-IcmX protochannel undergoes extensive rearrangements to form an extended transenvelope passage capable of transporting effector proteins from the bacterial cytoplasm into host cells as revealed by cryo-electron tomography. Collectively, our findings suggest that the DotA-IcmX complex functions as the gatekeeper for effector translocation of the Dot/Icm T4SS.
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Authors: Yue, J., Liu, J.
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In-situ structures of the Legionella Dot/Icm T4SS identify the DotA-IcmX complex as the gatekeeper for effector translocation.,Yue J, Heydari S, Park D, Chetrit D, Tachiyama S, Guo W, Botting JM, Wu S, Roy CR, Liu J bioRxiv [Preprint]. 2025 Jun 25:2025.06.23.660953. doi: , 10.1101/2025.06.23.660953. PMID:40666918<ref>PMID:40666918</ref>
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Description: In-situ cryo-EM structure of PR and DotA-IcmX of the Dot/Icm machine at C1
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Yue, J]]
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<div class="pdbe-citations 9nh0" style="background-color:#fffaf0;"></div>
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[[Category: Liu, J]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
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[[Category: Large Structures]]
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[[Category: Legionella pneumophila subsp. pneumophila]]
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[[Category: Liu J]]
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[[Category: Yue J]]

Current revision

In situ cryo-EM structure of PR and DotA-IcmX of the Legionella Dot/Icm T4SS machine at C1 symmetry

PDB ID 9nh0

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