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1vb2

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{{STRUCTURE_1vb2| PDB=1vb2 | SCENE= }}
{{STRUCTURE_1vb2| PDB=1vb2 | SCENE= }}
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'''T=1 capsid structure of Sesbania mosaic virus coat protein deletion mutant CP-N(delta)65-D146N-D149N'''
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===T=1 capsid structure of Sesbania mosaic virus coat protein deletion mutant CP-N(delta)65-D146N-D149N===
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==Overview==
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Sesbania mosaic virus particles consist of 180 coat protein subunits of 29kDa organized on a T=3 icosahedral lattice. N-terminal deletion mutants of coat protein that lack 36 (CP-NDelta36) and 65 (CP-NDelta65) residues from the N terminus, when expressed in Escherichia coli, produced similar T=1 capsids of approximate diameter 20nm. In contrast to the wild-type particles, these contain only 60 copies of the truncated protein subunits (T=1). CP-NDelta65 lacks the "beta-annulus" believed to be responsible for the error-free assembly of T=3 particles. Though the CP-NDelta36 mutant has the beta-annulus segment, it does not form a T=3 capsid, presumably because it lacks an arginine-rich motif found close to the amino terminus. Both CP-NDelta36 and CP-NDelta65 T=1 capsids retain many key features of the T=3 quaternary structure. Calcium binding geometries at the coat protein interfaces in these two particles are also nearly identical. When the conserved aspartate residues that coordinate the calcium, D146 and D149 in the CP-NDelta65, were mutated to asparagine (CP-NDelta65-D146N-D149N), the subunits assembled into T=1 particles but failed to bind calcium ions. The structure of this mutant revealed particles that were slightly expanded. The analysis of the structures of these mutant capsids suggests that although calcium binding contributes substantially to the stability of T=1 particles, it is not mandatory for their assembly. In contrast, the presence of a large fraction of the amino-terminal arm including sequences that precede the beta-annulus and the conserved D149 appear to be indispensable for the error-free assembly of T=3 particles.
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==About this Structure==
==About this Structure==
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[[Category: Icosahedral virus]]
[[Category: Icosahedral virus]]
[[Category: T=1 capsid]]
[[Category: T=1 capsid]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:19:10 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 09:02:41 2008''

Revision as of 06:02, 28 July 2008

Image:1vb2.png

Template:STRUCTURE 1vb2

T=1 capsid structure of Sesbania mosaic virus coat protein deletion mutant CP-N(delta)65-D146N-D149N

Template:ABSTRACT PUBMED 15342251

About this Structure

1VB2 is a Single protein structure of sequence from Sesbania mosaic virus. Full crystallographic information is available from OCA.

Reference

T=1 capsid structures of Sesbania mosaic virus coat protein mutants: determinants of T=3 and T=1 capsid assembly., Sangita V, Lokesh GL, Satheshkumar PS, Vijay CS, Saravanan V, Savithri HS, Murthy MR, J Mol Biol. 2004 Sep 17;342(3):987-99. PMID:15342251

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