1vd4

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{{STRUCTURE_1vd4| PDB=1vd4 | SCENE= }}
{{STRUCTURE_1vd4| PDB=1vd4 | SCENE= }}
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'''Solution structure of the zinc finger domain of TFIIE alpha'''
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===Solution structure of the zinc finger domain of TFIIE alpha===
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==Overview==
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The zinc finger domain in the large subunit of TFIIE (TFIIEalpha) is phylogenetically conserved and is essential for transcription. Here, we determined the solution structure of this domain by using NMR. It consisted of one alpha-helix and five beta-strands, showing novel features distinct from previously determined zinc-binding structures. We created point mutants of TFIIEalpha in this domain and examined their binding abilities to other general transcription factors as well as their transcription activities. Four Zn(2+)-ligand mutants, in which each of cysteine residues at positions 129, 132, 154, and 157 was replaced by alanine, possessed no transcription activities on a linearized template, whereas, on a supercoiled template, interesting functional asymmetry was observed: although the C-terminal two mutants abolished transcription activity (&lt;5%), the N-terminal two mutants retained about 20% activities. The N-terminal two mutants bound stronger to the small subunit of TFIIF than the wild type and the C-terminal two mutants were impaired in their binding abilities to the XPB subunits of TFIIH. These suggest that the structural integrity of the zinc finger domain is essential for the TFIIE function, particularly in the transition from the transcription initiation to elongation and the conformational tuning of this domain for appropriate positioning of TFIIF, TFIIH, and polymerase II would be needed depending on the situation and timing.
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{{ABSTRACT_PUBMED_15385556}}
==About this Structure==
==About this Structure==
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1VD4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VD4 OCA].
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1VD4 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1VD4 OCA].
==Reference==
==Reference==
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[[Category: Tanaka, A.]]
[[Category: Tanaka, A.]]
[[Category: Zinc finger]]
[[Category: Zinc finger]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:23:53 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 07:57:57 2008''

Revision as of 04:57, 28 July 2008

Image:1vd4.png

Template:STRUCTURE 1vd4

Solution structure of the zinc finger domain of TFIIE alpha

Template:ABSTRACT PUBMED 15385556

About this Structure

1VD4 is a Single protein structure of sequence from Homo sapiens. Full experimental information is available from OCA.

Reference

A novel zinc finger structure in the large subunit of human general transcription factor TFIIE., Okuda M, Tanaka A, Arai Y, Satoh M, Okamura H, Nagadoi A, Hanaoka F, Ohkuma Y, Nishimura Y, J Biol Chem. 2004 Dec 3;279(49):51395-403. Epub 2004 Sep 22. PMID:15385556

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