Sandbox Reserved 1844

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
m (Substrate included)
Line 20: Line 20:
=== Catalytic Triad ===
=== Catalytic Triad ===
 +
 +
As the PET substrate binds to the enzyme, its carbonyl bond
 +
attached to the first benzene ring must be harbored close to Serine in
 +
the catalytic triad (Fig. 2). Serine gets polarized by histidine, which is
 +
then stabilized by aspartic acid. Polarized Serine will attack the
 +
carbonyl bond (C-O) of the polyester, resulting in a tetrahedral in-
 +
termediate, which is then stabilized by the oxyanion hole. The hy-
 +
drolysis procedure is completed by a second nucleophilic attack
 +
mediated through a water molecule "PEThydrolase-insilico-engineering"
=== Ligand Binding Pocket ===
=== Ligand Binding Pocket ===
 +
hydrophobic pocket from "An engineered PET depolymerase to break down and recycle plastic bottles"
== Mutation Sites of Interest ==
== Mutation Sites of Interest ==

Revision as of 19:46, 27 March 2025

This Sandbox is Reserved from March 18 through September 1, 2025 for use in the course CH462 Biochemistry II taught by R. Jeremy Johnson and Mark Macbeth at the Butler University, Indianapolis, USA. This reservation includes Sandbox Reserved 1828 through Sandbox Reserved 1846.
To get started:
  • Click the edit this page tab at the top. Save the page after each step, then edit it again.
  • show the Scene authoring tools, create a molecular scene, and save it. Copy the green link into the page.
  • Add a description of your scene. Use the buttons above the wikitext box for bold, italics, links, headlines, etc.

More help: Help:Editing

Leaf Branch Compost Cutinase

caption

Drag the structure with the mouse to rotate

References

  1. ↑ Tournier V, Topham CM, Gilles A, David B, Folgoas C, Moya-Leclair E, Kamionka E, Desrousseaux ML, Texier H, Gavalda S, Cot M, Guemard E, Dalibey M, Nomme J, Cioci G, Barbe S, Chateau M, Andre I, Duquesne S, Marty A. An engineered PET depolymerase to break down and recycle plastic bottles. Nature. 2020 Apr;580(7802):216-219. doi: 10.1038/s41586-020-2149-4. Epub 2020 Apr, 8. PMID:32269349 doi:http://dx.doi.org/10.1038/s41586-020-2149-4

Student Contributors

Ashley Callaghan Rebecca Hoff Simone McCowan

Retrieved from "http://52.214.119.220/wiki/index.php/Sandbox_Reserved_1844"
Personal tools