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1yx5
From Proteopedia
(New page: 200px<br /> <applet load="1yx5" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yx5" /> '''Solution Structure of S5a UIM-1/Ubiquitin C...) |
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| - | [[Image:1yx5.gif|left|200px]]<br /> | + | [[Image:1yx5.gif|left|200px]]<br /><applet load="1yx5" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1yx5" size=" | + | |
caption="1yx5" /> | caption="1yx5" /> | ||
'''Solution Structure of S5a UIM-1/Ubiquitin Complex'''<br /> | '''Solution Structure of S5a UIM-1/Ubiquitin Complex'''<br /> | ||
==Overview== | ==Overview== | ||
| - | Ubiquitin is a key regulatory molecule in diverse cellular events. How | + | Ubiquitin is a key regulatory molecule in diverse cellular events. How cells determine the outcome of ubiquitylation remains unclear; however, a likely determinant is the specificity of ubiquitin receptor proteins for polyubiquitin chains of certain length and linkage. Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. Here, we report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules. This construct contains the two UIMs of S5a and we reveal their different ubiquitin-binding mechanisms and provide a rationale for their unique specificities for different ubiquitin-like domains. Furthermore, we provide direct evidence that S5a (196-306) binds either K63-linked or K48-linked polyubiquitin, and in both cases prefers longer chains. On the basis of these results we present a model for how S5a and other ubiquitin-binding proteins recognize polyubiquitin. |
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| + | ==Disease== | ||
| + | Known disease associated with this structure: Cleft palate, isolated OMIM:[[http://www.ncbi.nlm.nih.gov/entrez/dispomim.cgi?id=191339 191339]] | ||
==About this Structure== | ==About this Structure== | ||
| - | 1YX5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http:// | + | 1YX5 is a [http://en.wikipedia.org/wiki/Protein_complex Protein complex] structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YX5 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Walters, K | + | [[Category: Walters, K J.]] |
[[Category: Wang, Q.]] | [[Category: Wang, Q.]] | ||
[[Category: Young, P.]] | [[Category: Young, P.]] | ||
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[[Category: uim]] | [[Category: uim]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:10:00 2008'' |
Revision as of 14:10, 21 February 2008
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Solution Structure of S5a UIM-1/Ubiquitin Complex
Contents |
Overview
Ubiquitin is a key regulatory molecule in diverse cellular events. How cells determine the outcome of ubiquitylation remains unclear; however, a likely determinant is the specificity of ubiquitin receptor proteins for polyubiquitin chains of certain length and linkage. Proteasome subunit S5a contains two ubiquitin-interacting motifs (UIMs) through which it recruits ubiquitylated substrates to the proteasome for their degradation. Here, we report the structure of S5a (196-306) alone and complexed with two monoubiquitin molecules. This construct contains the two UIMs of S5a and we reveal their different ubiquitin-binding mechanisms and provide a rationale for their unique specificities for different ubiquitin-like domains. Furthermore, we provide direct evidence that S5a (196-306) binds either K63-linked or K48-linked polyubiquitin, and in both cases prefers longer chains. On the basis of these results we present a model for how S5a and other ubiquitin-binding proteins recognize polyubiquitin.
Disease
Known disease associated with this structure: Cleft palate, isolated OMIM:[191339]
About this Structure
1YX5 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of S5a bound to monoubiquitin provides a model for polyubiquitin recognition., Wang Q, Young P, Walters KJ, J Mol Biol. 2005 May 6;348(3):727-39. PMID:15826667
Page seeded by OCA on Thu Feb 21 16:10:00 2008
Categories: Homo sapiens | Protein complex | Walters, K J. | Wang, Q. | Young, P. | Nmr | Polyubiquitin | Proteasome | S5a | Uim
