9ntn
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Structure of Cap10-CdnD complex containing NDG modification== | |
| + | <StructureSection load='9ntn' size='340' side='right'caption='[[9ntn]], [[Resolution|resolution]] 2.43Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9ntn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Hyphomicrobiales Hyphomicrobiales]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9NTN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9NTN FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.43Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2KA:2-AMINO-4-OXO-4,7-DIHYDRO-3H-PYRROLO[2,3-D]PYRIMIDINE-5-CARBOXYLIC+ACID'>2KA</scene>, <scene name='pdbligand=COA:COENZYME+A'>COA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9ntn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9ntn OCA], [https://pdbe.org/9ntn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9ntn RCSB], [https://www.ebi.ac.uk/pdbsum/9ntn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9ntn ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | 7-deazapurines are nucleobase analogs essential for nucleic acid modifications in nearly all cellular life. Here, we discover a role for 7-deazapurines in protein modification within type IV CBASS anti-phage defense and define functions for CBASS ancillary proteins Cap9 and Cap10 in nucleobase-protein conjugation. A structure of Cap10 reveals a tRNA transglycosylase-family enzyme remodeled to bind the modified N-terminus of a partner cGAS/DncV-like nucleotidyltransferase linked to a 7-amido-7-deazaguanine (NDG) nucleobase. The structure of Cap9 explains how this QueC-like enzyme co-opts a 7-deazapurine biosynthetic reaction mechanism for NDG conjugation. We demonstrate that Cap9, Cap10, and NDG conjugation are essential for host defense against phage infection. Our results define a previously unknown 7-deazapurine protein modification and explain how nucleobase biosynthetic machinery has been repurposed for antiviral immunity. | ||
| - | + | Deazaguanylation is a nucleobase-protein conjugation required for type IV CBASS immunity.,Wassarman DR, Pfaff P, Paulo JA, Gygi SP, Shokat KM, Kranzusch PJ bioRxiv [Preprint]. 2025 Apr 6:2025.04.06.647259. doi: 10.1101/2025.04.06.647259. PMID:40236162<ref>PMID:40236162</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9ntn" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Hyphomicrobiales]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Gygi SP]] | ||
| + | [[Category: Kranzusch PJ]] | ||
| + | [[Category: Paulo JA]] | ||
| + | [[Category: Pfaff P]] | ||
| + | [[Category: Shokat KM]] | ||
| + | [[Category: Wassarman DR]] | ||
Current revision
Structure of Cap10-CdnD complex containing NDG modification
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