This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1vgl

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1vgl.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1vgl.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1vgl| PDB=1vgl | SCENE= }}
{{STRUCTURE_1vgl| PDB=1vgl | SCENE= }}
-
'''Crystal structure of tetrameric KaiB from T.elongatus BP-1'''
+
===Crystal structure of tetrameric KaiB from T.elongatus BP-1===
-
==Overview==
+
<!--
-
KaiB is a component of the circadian clock oscillator in cyanobacteria, which are the simplest organisms that exhibit circadian rhythms. KaiB consists of 108 amino-acid residues and has a molecular weight of 12 025 Da. KaiB and Cys-substituted KaiB mutants from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 were expressed as GST-fusion proteins in Escherichia coli, purified and crystallized. The crystals of wild-type KaiB belong to the monoclinic space group P2(1), with unit-cell parameters a = 89.6, b = 71.2, c = 106.8 A, beta = 100.1 degrees. While the native crystals diffract to 3.7 A, osmium derivatives, which show an approximately 4 A shrinkage in the b axis, diffract to 2.6 A. The crystals of the singly Cys-substituted mutant T64C with Hg, which show different morphology, diffract to 2.5 A and belong to the monoclinic space group P2, with unit-cell parameters a = 63.7, b = 33.4, c = 93.7 A, beta = 100.1 degrees. Anomalous difference Patterson maps of the Os- and Hg-derivative crystals had significant peaks in their Harker sections, suggesting that both derivatives are suitable for structure determination.
+
The line below this paragraph, {{ABSTRACT_PUBMED_16227211}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 16227211 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_16227211}}
==About this Structure==
==About this Structure==
Line 19: Line 23:
==Reference==
==Reference==
-
Crystallization and preliminary crystallographic analysis of the circadian clock protein KaiB from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1., Iwase R, Imada K, Hayashi F, Uzumaki T, Namba K, Ishiura M, Acta Crystallogr D Biol Crystallogr. 2004 Apr;60(Pt 4):727-9. Epub 2004, Mar 23. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15039567 15039567]
+
Functionally important substructures of circadian clock protein KaiB in a unique tetramer complex., Iwase R, Imada K, Hayashi F, Uzumaki T, Morishita M, Onai K, Furukawa Y, Namba K, Ishiura M, J Biol Chem. 2005 Dec 30;280(52):43141-9. Epub 2005 Oct 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16227211 16227211]
[[Category: Bacteria]]
[[Category: Bacteria]]
[[Category: Single protein]]
[[Category: Single protein]]
Line 29: Line 33:
[[Category: Uzumaki, T.]]
[[Category: Uzumaki, T.]]
[[Category: Circadian clock protein]]
[[Category: Circadian clock protein]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:31:07 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Jul 2 20:35:25 2008''

Revision as of 17:35, 2 July 2008

Image:1vgl.png

Template:STRUCTURE 1vgl

Crystal structure of tetrameric KaiB from T.elongatus BP-1

Template:ABSTRACT PUBMED 16227211

About this Structure

1VGL is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

Functionally important substructures of circadian clock protein KaiB in a unique tetramer complex., Iwase R, Imada K, Hayashi F, Uzumaki T, Morishita M, Onai K, Furukawa Y, Namba K, Ishiura M, J Biol Chem. 2005 Dec 30;280(52):43141-9. Epub 2005 Oct 13. PMID:16227211

Page seeded by OCA on Wed Jul 2 20:35:25 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1vgl"
Personal tools