Sandbox Reserved 1846
From Proteopedia
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=== F243 === | === F243 === | ||
- | + | The original side chain at position 243 is phenylalanine, which is located 3.6 Å from the ligand. Two mutations at this position, <scene name='10/1075247/Better_f243i/2'>F243I</scene> and F243W, increase the catalytic activity of the enzyme. The F243I mutation replaces phenylalanine with isoleucine, a smaller side chain that allows the ligand to sit closer. This reduces the ligand distance to 3.0 Å. This tighter interaction likely improves substrate binding. The F243W mutation introduces tryptophan, which has a bulkier, nitrogen-containing aromatic side chain. Tryptophan brings the ligand slightly closer at 3.2 Å and introduces potential for new interactions, such as hydrogen bonding or π-stacking. Both mutations result in improved catalytic performance. The F243I mutation leads to a 27.5% increase in activity, while the F243W mutation results in a 17.5% increase, compared to the wild-type enzyme. | |
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- | The original side chain at position 243 is phenylalanine, which is located 3.6 Å from the ligand. Two mutations at this | + | |
Revision as of 01:29, 15 April 2025
This Sandbox is Reserved from March 18 through September 1, 2025 for use in the course CH462 Biochemistry II taught by R. Jeremy Johnson and Mark Macbeth at the Butler University, Indianapolis, USA. This reservation includes Sandbox Reserved 1828 through Sandbox Reserved 1846. |
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Leaf Branch Compost Cutinase
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References
- ↑ 1.0 1.1 1.2 1.3 Tournier V, Topham CM, Gilles A, David B, Folgoas C, Moya-Leclair E, Kamionka E, Desrousseaux ML, Texier H, Gavalda S, Cot M, Guemard E, Dalibey M, Nomme J, Cioci G, Barbe S, Chateau M, Andre I, Duquesne S, Marty A. An engineered PET depolymerase to break down and recycle plastic bottles. Nature. 2020 Apr;580(7802):216-219. doi: 10.1038/s41586-020-2149-4. Epub 2020 Apr, 8. PMID:32269349 doi:http://dx.doi.org/10.1038/s41586-020-2149-4
- ↑ 2.0 2.1 2.2 2.3 2.4 Sui B, Wang T, Fang J, Hou Z, Shu T, Lu Z, Liu F, Zhu Y. Recent advances in the biodegradation of polyethylene terephthalate with cutinase-like enzymes. Front Microbiol. 2023 Oct 2;14:1265139. PMID:37849919 doi:10.3389/fmicb.2023.1265139
- ↑ Ueda H, Tabata J, Seshime Y, Masaki K, Sameshima-Yamashita Y, Kitamoto H. Cutinase-like biodegradable plastic-degrading enzymes from phylloplane yeasts have cutinase activity. Biosci Biotechnol Biochem. 2021 Jul 23;85(8):1890-1898. PMID:34160605 doi:10.1093/bbb/zbab113
- ↑ Kolattukudy PE. Biopolyester membranes of plants: cutin and suberin. Science. 1980 May 30;208(4447):990-1000. PMID:17779010 doi:10.1126/science.208.4447.990
- ↑ 5.0 5.1 5.2 5.3 5.4 Khairul Anuar NFS, Huyop F, Ur-Rehman G, Abdullah F, Normi YM, Sabullah MK, Abdul Wahab R. An Overview into Polyethylene Terephthalate (PET) Hydrolases and Efforts in Tailoring Enzymes for Improved Plastic Degradation. Int J Mol Sci. 2022 Oct 20;23(20):12644. PMID:36293501 doi:10.3390/ijms232012644
- ↑ 6.0 6.1 Burgin T, Pollard BC, Knott BC, Mayes HB, Crowley MF, McGeehan JE, Beckham GT, Woodcock HL. The reaction mechanism of the Ideonella sakaiensis PETase enzyme. Commun Chem. 2024 Mar 27;7(1):65. PMID:38538850 doi:10.1038/s42004-024-01154-x
- ↑ 7.0 7.1 Stevensen J, Janatunaim RZ, Ratnaputri AH, Aldafa SH, Rudjito RR, Saputro DH, Suhandono S, Putri RM, Aditama R, Fibriani A. Thermostability and Activity Improvements of PETase from Ideonella sakaiensis. ACS Omega. 2025 Feb 12;10(7):6385-6395. PMID:40028137 doi:10.1021/acsomega.4c05142
- ↑ Yoshida S, Hiraga K, Takehana T, Taniguchi I, Yamaji H, Maeda Y, Toyohara K, Miyamoto K, Kimura Y, Oda K. A bacterium that degrades and assimilates poly(ethylene terephthalate). Science. 2016 Mar 11;351(6278):1196-9. doi: 10.1126/science.aad6359. PMID:26965627 doi:http://dx.doi.org/10.1126/science.aad6359
- ↑ Landrigan PJ, Stegeman JJ, Fleming LE, Allemand D, Anderson DM, Backer LC, Brucker-Davis F, Chevalier N, Corra L, Czerucka D, Bottein MD, Demeneix B, Depledge M, Deheyn DD, Dorman CJ, Fénichel P, Fisher S, Gaill F, Galgani F, Gaze WH, Giuliano L, Grandjean P, Hahn ME, Hamdoun A, Hess P, Judson B, Laborde A, McGlade J, Mu J, Mustapha A, Neira M, Noble RT, Pedrotti ML, Reddy C, Rocklöv J, Scharler UM, Shanmugam H, Taghian G, van de Water JAJM, Vezzulli L, Weihe P, Zeka A, Raps H, Rampal P. Human Health and Ocean Pollution. Ann Glob Health. 2020 Dec 3;86(1):151. PMID:33354517 doi:10.5334/aogh.2831
- ↑ Jambeck JR, Geyer R, Wilcox C, Siegler TR, Perryman M, Andrady A, Narayan R, Law KL. Marine pollution. Plastic waste inputs from land into the ocean. Science. 2015 Feb 13;347(6223):768-71. PMID:25678662 doi:10.1126/science.1260352
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