9qtu
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==human PAN2-PAN3 deadenylase complex in the apo state== | |
| + | <StructureSection load='9qtu' size='340' side='right'caption='[[9qtu]], [[Resolution|resolution]] 4.60Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[9qtu]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9QTU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9QTU FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 4.6Å</td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9qtu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9qtu OCA], [https://pdbe.org/9qtu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9qtu RCSB], [https://www.ebi.ac.uk/pdbsum/9qtu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9qtu ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/PAN3_HUMAN PAN3_HUMAN] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The lifespan of most eukaryotic mRNAs is modulated by the gradual shortening of the poly(A) tail and removal of the associated poly(A)-binding protein. The human PAN2-PAN3 complex catalyzes initial deadenylation by shortening long poly(A) tails associated with PABPC1. Both PAN2-PAN3 and PABPC1 are evolutionarily conserved from fungi to humans. How the human complex has adapted to recognize and act on longer poly(A) tails characteristic of mammalian mRNAs remains unclear. Here, we report a method to obtain homo-polymeric poly(A) RNAs up to 240 nt, mimicking the synthesis length of poly(A) tails in mammals. We recapitulate human deadenylation properties in vitro, with PAN2-PAN3 showing greater activity on long poly(A)-PABPC1 ribonucleoprotein substrates. Single-particle cryo-electron microscopy (cryo-EM) analyses of PAN2-PAN3 bound to poly(A)-PABPC1 ribonucleoproteins uncover a longer substrate-binding path in the case of the human deadenylase compared to fungi. Altogether, these data provide a rationale for the co-evolution of deadenylase properties and poly(A) tail lengths. | ||
| - | + | Mechanisms governing poly(A)-tail-length specificity of the human PAN2-PAN3 deadenylase complex.,Albrecht JC, Reitinger T, Basquin J, Schussler S, Riggi M, Schafer IB, Conti E Cell Rep. 2025 Nov 22;44(12):116609. doi: 10.1016/j.celrep.2025.116609. PMID:41275497<ref>PMID:41275497</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 9qtu" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Albrecht JC]] | ||
| + | [[Category: Basquin J]] | ||
| + | [[Category: Conti E]] | ||
| + | [[Category: Reitinger T]] | ||
| + | [[Category: Schaefer IB]] | ||
| + | [[Category: Schuessler S]] | ||
Current revision
human PAN2-PAN3 deadenylase complex in the apo state
| |||||||||||
