User:Peyton Jenkins/Sandbox 1
From Proteopedia
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== Structural Highlights == | == Structural Highlights == | ||
=== STK11 === | === STK11 === | ||
| - | [[STK11]] can be broken down into 3 domains. An N-terminal domain (aa 1-42), kinase domain (aa 43-347), and a C-terminal domain (aa 348-433). The activation loop of [[STK11]] is located from residues ~202-212. Within the activation loop is P204, which interacts with a hydrophobic pocket on MO25, which is necessary to stabilize the active conformation. D98 forms a salt bridge with K78, further stabilizing the active site. R74 hydrogen bonds with Q251 of STRADα to stabilize the interaction between the two proteins. | + | [[STK11]] can be broken down into 3 domains. An N-terminal domain (aa 1-42), kinase domain (aa 43-347), and a C-terminal domain (aa 348-433). The activation loop of [[STK11]] is located from residues ~202-212. Within the activation loop is P204, which interacts with a hydrophobic pocket on MO25, which is necessary to stabilize the active conformation. D98 forms a salt bridge with K78, further stabilizing the active site. R74 hydrogen bonds with Q251 of STRADα to stabilize the interaction between the two proteins. The β2-β3 loop and β7-β8 sheets of STK11 also interact with STRADα. In the β2-β3 loop R74 hydrogen bonds with Q251 of STRADα. |
=== STRADα === | === STRADα === | ||
| - | The WEF motif (aa 429-431) on the C-terminus of STRADα | + | STRAD alpha is composed of 2 domains, an N-terminal domain (aa 1-58) and pseudokinase domain (aa 59-347). STRADα binds STK11 through its pseudokinase domain, with the activation loop interacting with the the β2-β3 loop and β7-β8 sheets of STK11. The αC of STRADα interacts with the surface of MO25, further stabilizing the interaction between proteins. Additionally there is a WEF motif (aa 429-431) on the C-terminus of STRADα interacting with the C-terminus of MO25. |
| + | === MO25 === | ||
| + | MO25 is a repeat of α-helices spanning the entire length of the protein. Residues R240 and F243 interact with the A205 and A206 of the STK11 activation loop to further stabilize the active conformation. | ||
== References == | == References == | ||
<references/> | <references/> | ||
Revision as of 01:39, 29 April 2025
2WTK: Hetertrimeric Complex of STK11, MO25, and STRADα
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