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1w0n

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{{STRUCTURE_1w0n| PDB=1w0n | SCENE= }}
{{STRUCTURE_1w0n| PDB=1w0n | SCENE= }}
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'''STRUCTURE OF UNCOMPLEXED CARBOHYDRATE BINDING DOMAIN CBM36'''
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===STRUCTURE OF UNCOMPLEXED CARBOHYDRATE BINDING DOMAIN CBM36===
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==Overview==
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The enzymatic degradation of polysaccharides harnesses multimodular enzymes whose carbohydrate binding modules (CBM) target the catalytic domain onto the recalcitrant substrate. Here we report the ab initio structure determination and subsequent refinement, at 0.8 A resolution, of the CBM36 domain of the Paenibacillus polymyxa xylanase 43A. Affinity electrophoresis, isothermal titration calorimetry, and UV difference spectroscopy demonstrate that CBM36 is a novel Ca(2+)-dependent xylan binding domain. The 3D structure of CBM36 in complex with xylotriose and Ca(2+), at 1.5 A resolution, displays significant conformational changes compared to the native structure and reveals the molecular basis for its unique Ca(2+)-dependent binding of xylooligosaccharides through coordination of the O2 and O3 hydroxyls. CBM36 is one of an emerging spectrum of carbohydrate binding modules that increasingly find applications in industry and display great potential for mapping the "glyco-architecture" of plant cells.
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{{ABSTRACT_PUBMED_15242594}}
==About this Structure==
==About this Structure==
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[[Category: Xylan]]
[[Category: Xylan]]
[[Category: Xylanase]]
[[Category: Xylanase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 12:59:59 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:10:48 2008''

Revision as of 13:10, 28 July 2008

Image:1w0n.png

Template:STRUCTURE 1w0n

STRUCTURE OF UNCOMPLEXED CARBOHYDRATE BINDING DOMAIN CBM36

Template:ABSTRACT PUBMED 15242594

About this Structure

1W0N is a Single protein structure of sequence from Paenibacillus polymyxa. Full crystallographic information is available from OCA.

Reference

Ab initio structure determination and functional characterization of CBM36; a new family of calcium-dependent carbohydrate binding modules., Jamal-Talabani S, Boraston AB, Turkenburg JP, Tarbouriech N, Ducros VM, Davies GJ, Structure. 2004 Jul;12(7):1177-87. PMID:15242594

Page seeded by OCA on Mon Jul 28 16:10:48 2008

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