1b5e

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(New page: 200px<br /> <applet load="1b5e" size="450" color="white" frame="true" align="right" spinBox="true" caption="1b5e, resolution 1.60&Aring;" /> '''DCMP HYDROXYMETHYLA...)
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==About this Structure==
==About this Structure==
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1B5E is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/ ]] with DCM as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/ ]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.2.8 2.1.2.8]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B5E OCA]].
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1B5E is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Enterobacteria_phage_t2 Enterobacteria phage t2]] with DCM as [[http://en.wikipedia.org/wiki/ligand ligand]]. Active as [[http://en.wikipedia.org/wiki/Deoxycytidylate_5-hydroxymethyltransferase Deoxycytidylate 5-hydroxymethyltransferase]], with EC number [[http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.2.8 2.1.2.8]]. Structure known Active Site: CAT. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1B5E OCA]].
==Reference==
==Reference==
Crystal structure of deoxycytidylate hydroxymethylase from bacteriophage T4, a component of the deoxyribonucleoside triphosphate-synthesizing complex., Song HK, Sohn SH, Suh SW, EMBO J. 1999 Mar 1;18(5):1104-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10064578 10064578]
Crystal structure of deoxycytidylate hydroxymethylase from bacteriophage T4, a component of the deoxyribonucleoside triphosphate-synthesizing complex., Song HK, Sohn SH, Suh SW, EMBO J. 1999 Mar 1;18(5):1104-13. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=10064578 10064578]
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[[Category: Deoxycytidylate 5-hydroxymethyltransferase]]
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[[Category: Enterobacteria phage t2]]
[[Category: Single protein]]
[[Category: Single protein]]
[[Category: Sohn, S.H.]]
[[Category: Sohn, S.H.]]
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[[Category: hydroxymethylase]]
[[Category: hydroxymethylase]]
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Oct 29 18:41:46 2007''
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''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:00:50 2007''

Revision as of 09:56, 30 October 2007


1b5e, resolution 1.60Å

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DCMP HYDROXYMETHYLASE FROM T4

Overview

Bacteriophage T4 deoxycytidylate hydroxymethylase (EC 2.1.2.8), a, homodimer of 246-residue subunits, catalyzes hydroxymethylation of the, cytosine base in deoxycytidylate (dCMP) to produce 5-hydroxymethyl-dCMP., It forms part of a phage DNA protection system and appears to function in, vivo as a component of a multienzyme complex called deoxyribonucleoside, triphosphate (dNTP) synthetase. We have determined its crystal structure, in the presence of the substrate dCMP at 1.6 A resolution. The structure, reveals a subunit fold and a dimerization pattern in common with, thymidylate synthases, despite low (approximately 20%) sequence identity., Among the residues that form the dCMP binding site, those interacting with, the sugar and phosphate are arranged in a configuration similar to the, ... [(full description)]

About this Structure

1B5E is a [Single protein] structure of sequence from [Enterobacteria phage t2] with DCM as [ligand]. Active as [Deoxycytidylate 5-hydroxymethyltransferase], with EC number [2.1.2.8]. Structure known Active Site: CAT. Full crystallographic information is available from [OCA].

Reference

Crystal structure of deoxycytidylate hydroxymethylase from bacteriophage T4, a component of the deoxyribonucleoside triphosphate-synthesizing complex., Song HK, Sohn SH, Suh SW, EMBO J. 1999 Mar 1;18(5):1104-13. PMID:10064578

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