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1w8y

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[[Image:1w8y.gif|left|200px]]
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{{STRUCTURE_1w8y| PDB=1w8y | SCENE= }}
{{STRUCTURE_1w8y| PDB=1w8y | SCENE= }}
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'''CRYSTAL STRUCTURE OF THE NITROCEFIN ACYL-DD-PEPTIDASE FROM ACTINOMADURA R39.'''
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===CRYSTAL STRUCTURE OF THE NITROCEFIN ACYL-DD-PEPTIDASE FROM ACTINOMADURA R39.===
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==Overview==
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Actinomadura sp. R39 produces an exocellular DD-peptidase/penicillin-binding protein (PBP) whose primary structure is similar to that of Escherichia coli PBP4. It is characterized by a high beta-lactam-binding activity (second order rate constant for the acylation of the active site serine by benzylpenicillin: k2/K = 300 mm(-1) s(-1)). The crystal structure of the DD-peptidase from Actinomadura R39 was solved at a resolution of 1.8 angstroms by single anomalous dispersion at the cobalt resonance wavelength. The structure is composed of three domains: a penicillin-binding domain similar to the penicillin-binding domain of E. coli PBP5 and two domains of unknown function. In most multimodular PBPs, additional domains are generally located at the C or N termini of the penicillin-binding domain. In R39, the other two domains are inserted in the penicillin-binding domain, between the SXXK and SXN motifs, in a manner similar to "Matryoshka dolls." One of these domains is composed of a five-stranded beta-sheet with two helices on one side, and the other domain is a double three-stranded beta-sheet inserted in the previous domain. Additionally, the 2.4-angstroms structure of the acyl-enzyme complex of R39 with nitrocefin reveals the absence of active site conformational change upon binding the beta-lactams.
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{{ABSTRACT_PUBMED_15987687}}
==About this Structure==
==About this Structure==
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[[Category: Sauvage, E.]]
[[Category: Sauvage, E.]]
[[Category: Penicillin-binding,actinomadura,peptidoglycan,nitrocefin]]
[[Category: Penicillin-binding,actinomadura,peptidoglycan,nitrocefin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:19:21 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 03:37:22 2008''

Revision as of 00:37, 29 July 2008

Image:1w8y.png

Template:STRUCTURE 1w8y

CRYSTAL STRUCTURE OF THE NITROCEFIN ACYL-DD-PEPTIDASE FROM ACTINOMADURA R39.

Template:ABSTRACT PUBMED 15987687

About this Structure

1W8Y is a Single protein structure of sequence from Actinomadura. Full crystallographic information is available from OCA.

Reference

Crystal structure of the Actinomadura R39 DD-peptidase reveals new domains in penicillin-binding proteins., Sauvage E, Herman R, Petrella S, Duez C, Bouillenne F, Frere JM, Charlier P, J Biol Chem. 2005 Sep 2;280(35):31249-56. Epub 2005 Jun 29. PMID:15987687

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