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1wa5

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[[Image:1wa5.gif|left|200px]]
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[[Image:1wa5.png|left|200px]]
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==Overview==
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{{ABSTRACT_15602554}}
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The nuclear import and export of macromolecular cargoes through nuclear pore complexes is mediated primarily by carriers such as importin-beta. Importins carry cargoes into the nucleus, whereas exportins carry cargoes to the cytoplasm. Transport is orchestrated by nuclear RanGTP, which dissociates cargoes from importins, but conversely is required for cargo binding to exportins. Here we present the 2.0 A crystal structure of the nuclear export complex formed by exportin Cse1p complexed with its cargo (Kap60p) and RanGTP, thereby providing a structural framework for understanding nuclear protein export and the different functions of RanGTP in export and import. In the complex, Cse1p coils around both RanGTP and Kap60p, stabilizing the RanGTP-state and clamping the Kap60p importin-beta-binding domain, ensuring that only cargo-free Kap60p is exported. Mutagenesis indicated that conformational changes in exportins couple cargo binding to high affinity for RanGTP, generating a spring-loaded molecule to facilitate disassembly of the export complex following GTP hydrolysis in the cytoplasm.
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==About this Structure==
==About this Structure==
1WA5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The following page contains interesting information on the relation of 1WA5 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html Importins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WA5 OCA].
1WA5 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Canis_lupus_familiaris Canis lupus familiaris] and [http://en.wikipedia.org/wiki/Saccharomyces_cerevisiae Saccharomyces cerevisiae]. The following page contains interesting information on the relation of 1WA5 with [[http://pdb.rcsb.org/pdb/static.do?p=education_discussion/molecule_of_the_month/pdb85_1.html Importins]]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WA5 OCA].
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==Reference==
 
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Structural basis for the assembly of a nuclear export complex., Matsuura Y, Stewart M, Nature. 2004 Dec 16;432(7019):872-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15602554 15602554]
 
[[Category: Canis lupus familiaris]]
[[Category: Canis lupus familiaris]]
[[Category: Importins]]
[[Category: Importins]]
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[[Category: Protein transport]]
[[Category: Protein transport]]
[[Category: Ran gtpase]]
[[Category: Ran gtpase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:22:17 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Jun 26 18:06:07 2008''

Revision as of 15:06, 26 June 2008

Image:1wa5.png

Template:STRUCTURE 1wa5

CRYSTAL STRUCTURE OF THE EXPORTIN CSE1P COMPLEXED WITH ITS CARGO (KAP60P) AND RANGTP


Template:ABSTRACT 15602554

About this Structure

1WA5 is a Protein complex structure of sequences from Canis lupus familiaris and Saccharomyces cerevisiae. The following page contains interesting information on the relation of 1WA5 with [Importins]. Full crystallographic information is available from OCA.

Page seeded by OCA on Thu Jun 26 18:06:07 2008

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