1wbp

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{{STRUCTURE_1wbp| PDB=1wbp | SCENE= }}
{{STRUCTURE_1wbp| PDB=1wbp | SCENE= }}
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'''SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE'''
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===SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE===
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==Overview==
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The arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is phosphorylated by SR protein kinases (SRPKs) and Clk/Sty kinases. However, the mode of phosphorylation by these kinases and their coordination in the biological regulation of ASF/SF2 is unknown. Here, we report the crystal structure of an active fragment of human SRPK1 bound to a peptide derived from an SR protein. This structure led us to identify a docking motif in ASF/SF2. We find that this docking motif restricts phosphorylation of ASF/SF2 by SRPK1 to the N-terminal part of the RS domain - a property essential for its assembly into nuclear speckles. We further show that Clk/Sty causes release of ASF/SF2 from speckles by phosphorylating the C-terminal part of its RS domain. These results suggest that the docking motif of ASF/SF2 is a key regulatory element for sequential phosphorylation by SRPK1 and Clk/Sty and, thus, is essential for its subcellular localization.
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{{ABSTRACT_PUBMED_16209947}}
==About this Structure==
==About this Structure==
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[[Category: Srpk]]
[[Category: Srpk]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:25:52 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:23:06 2008''

Revision as of 10:23, 28 July 2008

Image:1wbp.png

Template:STRUCTURE 1wbp

SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE

Template:ABSTRACT PUBMED 16209947

About this Structure

1WBP is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2., Ngo JC, Chakrabarti S, Ding JH, Velazquez-Dones A, Nolen B, Aubol BE, Adams JA, Fu XD, Ghosh G, Mol Cell. 2005 Oct 7;20(1):77-89. PMID:16209947

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