1wd4

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{{STRUCTURE_1wd4| PDB=1wd4 | SCENE= }}
{{STRUCTURE_1wd4| PDB=1wd4 | SCENE= }}
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'''Crystal structure of arabinofuranosidase complexed with arabinose'''
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===Crystal structure of arabinofuranosidase complexed with arabinose===
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==Overview==
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As the first known structures of a glycoside hydrolase family 54 (GH54) enzyme, we determined the crystal structures of free and arabinose-complex forms of Aspergillus kawachii IFO4308 alpha-l-arabinofuranosidase (AkAbfB). AkAbfB comprises two domains: a catalytic domain and an arabinose-binding domain (ABD). The catalytic domain has a beta-sandwich fold similar to those of clan-B glycoside hydrolases. ABD has a beta-trefoil fold similar to that of carbohydrate-binding module (CBM) family 13. However, ABD shows a number of characteristics distinctive from those of CBM family 13, suggesting that it could be classified into a new CBM family. In the arabinose-complex structure, one of three arabinofuranose molecules is bound to the catalytic domain through many interactions. Interestingly, a disulfide bond formed between two adjacent cysteine residues recognized the arabinofuranose molecule in the active site. From the location of this arabinofuranose and the results of a mutational study, the nucleophile and acid/base residues were determined to be Glu(221) and Asp(297), respectively. The other two arabinofuranose molecules are bound to ABD. The O-1 atoms of the two arabinofuranose molecules bound at ABD are both pointed toward the solvent, indicating that these sites can both accommodate an arabinofuranose side-chain moiety linked to decorated arabinoxylans.
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(as it appears on PubMed at http://www.pubmed.gov), where 15292273 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15292273}}
==About this Structure==
==About this Structure==
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[[Category: Beta-trefoil]]
[[Category: Beta-trefoil]]
[[Category: Complex with arabinose]]
[[Category: Complex with arabinose]]
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Revision as of 10:41, 28 July 2008

Image:1wd4.png

Template:STRUCTURE 1wd4

Crystal structure of arabinofuranosidase complexed with arabinose

Template:ABSTRACT PUBMED 15292273

About this Structure

1WD4 is a Single protein structure of sequence from Aspergillus kawachii. Full crystallographic information is available from OCA.

Reference

Crystal structure of a family 54 alpha-L-arabinofuranosidase reveals a novel carbohydrate-binding module that can bind arabinose., Miyanaga A, Koseki T, Matsuzawa H, Wakagi T, Shoun H, Fushinobu S, J Biol Chem. 2004 Oct 22;279(43):44907-14. Epub 2004 Aug 3. PMID:15292273

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