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| - | [[Image:1wgt.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1wgt| PDB=1wgt | SCENE= }} | | {{STRUCTURE_1wgt| PDB=1wgt | SCENE= }} |
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| - | '''X-RAY STRUCTURE OF WHEAT GERM AGGLUTININ ISOLECTIN 3'''
| + | ===X-RAY STRUCTURE OF WHEAT GERM AGGLUTININ ISOLECTIN 3=== |
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| - | ==Overview==
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| - | Wheat germ agglutinin isolectin 3 (WGA3) was crystallized from 10 mM acetate buffer at pH 4.9 containing 6 mM CaCl(2) and 4%(v/v) ethanol. The crystal belongs to monoclinic space group P2(1) with unit-cell dimensions a = 44.86, b = 91.02, c = 44.86 A, and beta = 110.22 degrees. The asymmetric unit contains two molecules (V(m) = 2.51 A(3) Da(-1)). The crystal structure was solved by the molecular-replacement method and was refined by the simulated-annealing method. The conventional R value was 0.191 for 19713 reflections [|F(o)| > 3sigma(F)] in the resolution range 8-1.9 A. The r.m.s. deviations from the ideal bond distances and angles were 0.014 A, and 3.0 degrees, respectively, and the estimated coordinate error was 0.2-0.25 A. The two molecules in the asymmetric unit are related by the pseudo twofold symmetry and form a dimer structure. The backbone structures of the two subunits are nearly identical with the r.m.s. difference of 0.36 A for the superposition of equivalent C(alpha) atoms. The dimer structure is very similar to those of isolectins 1 and 2 with the r.m.s. difference of 0.35-0.39 A for the C(alpha) superposition. Since amino-acid residues which differ from those of isolectin 1 or 2 are not involved in the contact between the two subunits, the subunit-subunit interaction is not significantly affected by the replacement of these residues. As a result, the geometry of the sugar-binding sites which are located at the interface between the two subunit molecules is basically conserved among three isolectins.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15299769}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15299769 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15299769}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Jigami, Y.]] | | [[Category: Jigami, Y.]] |
| | [[Category: Nagahora, H.]] | | [[Category: Nagahora, H.]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:38:46 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:01:37 2008'' |
Revision as of 09:01, 29 July 2008
Template:STRUCTURE 1wgt
X-RAY STRUCTURE OF WHEAT GERM AGGLUTININ ISOLECTIN 3
Template:ABSTRACT PUBMED 15299769
About this Structure
1WGT is a Single protein structure of sequence from Triticum aestivum. Full crystallographic information is available from OCA.
Reference
X-ray structure of wheat germ agglutinin isolectin 3., Harata K, Nagahora H, Jigami Y, Acta Crystallogr D Biol Crystallogr. 1995 Nov 1;51(Pt 6):1013-9. PMID:15299769
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