9sh8
From Proteopedia
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- | '''Unreleased structure''' | ||
- | + | ==Thermus thermophilus asparaginyl-tRNA synthetase with bound sulphamoyl-analogue of asparaginyl-adenylate (AsnAMS)== | |
+ | <StructureSection load='9sh8' size='340' side='right'caption='[[9sh8]], [[Resolution|resolution]] 2.62Å' scene=''> | ||
+ | == Structural highlights == | ||
+ | <table><tr><td colspan='2'>[[9sh8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB8 Thermus thermophilus HB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=9SH8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=9SH8 FirstGlance]. <br> | ||
+ | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.62Å</td></tr> | ||
+ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NSS:5-O-[N-(L-ASPARAGINYL)SULFAMOYL]ADENOSINE'>NSS</scene></td></tr> | ||
+ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=9sh8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=9sh8 OCA], [https://pdbe.org/9sh8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=9sh8 RCSB], [https://www.ebi.ac.uk/pdbsum/9sh8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=9sh8 ProSAT]</span></td></tr> | ||
+ | </table> | ||
+ | == Function == | ||
+ | [https://www.uniprot.org/uniprot/SYN_THET8 SYN_THET8] | ||
+ | <div style="background-color:#fffaf0;"> | ||
+ | == Publication Abstract from PubMed == | ||
+ | The crystal structure of Thermus thermophilus asparaginyl-tRNA synthetase has been solved by multiple isomorphous replacement and refined at 2.6 A resolution. This is the last of the three class IIb aminoacyl-tRNA synthetase structures to be determined. As expected from primary sequence comparisons, there are remarkable similarities between the tertiary structures of asparaginyl-tRNA synthetase and aspartyl-tRNA synthetase, and most of the active site residues are identical except for three key differences. The structure at 2.65 A of asparaginyl-tRNA synthetase complexed with a non-hydrolysable analogue of asparaginyl-adenylate permits a detailed explanation of how these three differences allow each enzyme to discriminate between their respective and very similar amino acid substrates, asparagine and aspartic acid. In addition, a structure of the complex of asparaginyl-tRNA synthetase with ATP shows exactly the same configuration of three divalent cations as previously observed in the seryl-tRNA synthetase-ATP complex, showing that this a general feature of class II synthetases. The structural similarity of asparaginyl- and aspartyl-tRNA synthetases as well as that of both enzymes to the ammonia-dependent asparagine synthetase suggests that these three enzymes have evolved relatively recently from a common ancestor. | ||
- | + | The crystal structure of asparaginyl-tRNA synthetase from Thermus thermophilus and its complexes with ATP and asparaginyl-adenylate: the mechanism of discrimination between asparagine and aspartic acid.,Berthet-Colominas C, Seignovert L, Hartlein M, Grotli M, Cusack S, Leberman R EMBO J. 1998 May 15;17(10):2947-60. PMID:9582288<ref>PMID:9582288</ref> | |
- | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
- | [[Category: | + | </div> |
- | [[Category: Berthet-Colominas | + | <div class="pdbe-citations 9sh8" style="background-color:#fffaf0;"></div> |
- | [[Category: Cusack | + | == References == |
- | [[Category: Leberman | + | <references/> |
- | [[Category: Seignovert | + | __TOC__ |
+ | </StructureSection> | ||
+ | [[Category: Large Structures]] | ||
+ | [[Category: Thermus thermophilus HB8]] | ||
+ | [[Category: Berthet-Colominas C]] | ||
+ | [[Category: Cusack S]] | ||
+ | [[Category: Leberman R]] | ||
+ | [[Category: Seignovert L]] |
Current revision
Thermus thermophilus asparaginyl-tRNA synthetase with bound sulphamoyl-analogue of asparaginyl-adenylate (AsnAMS)
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