1wj2

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1wj2.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1wj2.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1wj2| PDB=1wj2 | SCENE= }}
{{STRUCTURE_1wj2| PDB=1wj2 | SCENE= }}
-
'''Solution Structure of the C-terminal WRKY Domain of AtWRKY4'''
+
===Solution Structure of the C-terminal WRKY Domain of AtWRKY4===
-
==Overview==
+
<!--
-
The WRKY proteins comprise a major family of transcription factors that are essential in pathogen and salicylic acid responses of higher plants as well as a variety of plant-specific reactions. They share a DNA binding domain, designated as the WRKY domain, which contains an invariant WRKYGQK sequence and a CX4-5CX22-23HXH zinc binding motif. Herein, we report the NMR solution structure of the C-terminal WRKY domain of the Arabidopsis thaliana WRKY4 protein. The structure consists of a four-stranded beta-sheet, with a zinc binding pocket formed by the conserved Cys/His residues located at one end of the beta-sheet, revealing a novel zinc and DNA binding structure. The WRKYGQK residues correspond to the most N-terminal beta-strand, kinked in the middle of the sequence by the Gly residue, which enables extensive hydrophobic interactions involving the Trp residue and contributes to the structural stability of the beta-sheet. Based on a profile of NMR chemical shift perturbations, we propose that the same strand enters the DNA groove and forms contacts with the DNA bases.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15705956}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15705956 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15705956}}
==About this Structure==
==About this Structure==
-
1WJ2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WJ2 OCA].
+
1WJ2 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WJ2 OCA].
==Reference==
==Reference==
Line 32: Line 36:
[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Zinc-binding]]
[[Category: Zinc-binding]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:44:56 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:48:13 2008''

Revision as of 12:48, 28 July 2008

Image:1wj2.png

Template:STRUCTURE 1wj2

Solution Structure of the C-terminal WRKY Domain of AtWRKY4

Template:ABSTRACT PUBMED 15705956

About this Structure

1WJ2 is a Single protein structure of sequence from Arabidopsis thaliana. Full experimental information is available from OCA.

Reference

Solution structure of an Arabidopsis WRKY DNA binding domain., Yamasaki K, Kigawa T, Inoue M, Tateno M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Hayami N, Terada T, Shirouzu M, Tanaka A, Seki M, Shinozaki K, Yokoyama S, Plant Cell. 2005 Mar;17(3):944-56. Epub 2005 Feb 10. PMID:15705956

Page seeded by OCA on Mon Jul 28 15:48:13 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1wj2"
Personal tools