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1wle

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[[Image:1wle.gif|left|200px]]
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{{STRUCTURE_1wle| PDB=1wle | SCENE= }}
{{STRUCTURE_1wle| PDB=1wle | SCENE= }}
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'''Crystal Structure of mammalian mitochondrial seryl-tRNA synthetase complexed with seryl-adenylate'''
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===Crystal Structure of mammalian mitochondrial seryl-tRNA synthetase complexed with seryl-adenylate===
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==Overview==
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The secondary structures of metazoan mitochondrial (mt) tRNAs(Ser) deviate markedly from the paradigm of the canonical cloverleaf structure; particularly, tRNA(Ser)(GCU) corresponding to the AGY codon (Y=U and C) is highly truncated and intrinsically missing the entire dihydrouridine arm. None of the mt serine isoacceptors possesses the elongated variable arm, which is the universal landmark for recognition by seryl-tRNA synthetase (SerRS). Here, we report the crystal structure of mammalian mt SerRS from Bos taurus in complex with seryl adenylate at an atomic resolution of 1.65 A. Coupling structural information with a tRNA-docking model and the mutagenesis studies, we have unraveled the key elements that establish tRNA binding specificity, differ from all other known bacterial and eukaryotic systems, are the characteristic extensions in both extremities, as well as a few basic residues residing in the amino-terminal helical arm of mt SerRS. Our data further uncover an unprecedented mechanism of a dual-mode recognition employed to discriminate two distinct 'bizarre' mt tRNAs(Ser) by alternative combination of interaction sites.
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The line below this paragraph, {{ABSTRACT_PUBMED_16163389}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16163389 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16163389}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Dual-mode recognition of noncanonical tRNAs(Ser) by seryl-tRNA synthetase in mammalian mitochondria., Chimnaronk S, Gravers Jeppesen M, Suzuki T, Nyborg J, Watanabe K, EMBO J. 2005 Oct 5;24(19):3369-79. Epub 2005 Sep 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16163389 16163389]
Dual-mode recognition of noncanonical tRNAs(Ser) by seryl-tRNA synthetase in mammalian mitochondria., Chimnaronk S, Gravers Jeppesen M, Suzuki T, Nyborg J, Watanabe K, EMBO J. 2005 Oct 5;24(19):3369-79. Epub 2005 Sep 15. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16163389 16163389]
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Characterization and tRNA recognition of mammalian mitochondrial seryl-tRNA synthetase., Yokogawa T, Shimada N, Takeuchi N, Benkowski L, Suzuki T, Omori A, Ueda T, Nishikawa K, Spremulli LL, Watanabe K, J Biol Chem. 2000 Jun 30;275(26):19913-20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/10764807 10764807]
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Dual mode recognition of two isoacceptor tRNAs by mammalian mitochondrial seryl-tRNA synthetase., Shimada N, Suzuki T, Watanabe K, J Biol Chem. 2001 Dec 14;276(50):46770-8. Epub 2001 Sep 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/11577083 11577083]
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Crystallization and preliminary X-ray diffraction study of mammalian mitochondrial seryl-tRNA synthetase., Chimnaronk S, Jeppesen MG, Shimada N, Suzuki T, Nyborg J, Watanabe K, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1319-22. Epub 2004, Jun 22. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15213404 15213404]
[[Category: Bos taurus]]
[[Category: Bos taurus]]
[[Category: Serine--tRNA ligase]]
[[Category: Serine--tRNA ligase]]
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[[Category: Watanabe, K.]]
[[Category: Watanabe, K.]]
[[Category: Ligase]]
[[Category: Ligase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:50:15 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 13:09:39 2008''

Revision as of 10:09, 28 July 2008

Image:1wle.png

Template:STRUCTURE 1wle

Crystal Structure of mammalian mitochondrial seryl-tRNA synthetase complexed with seryl-adenylate

Template:ABSTRACT PUBMED 16163389

About this Structure

1WLE is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.

Reference

Dual-mode recognition of noncanonical tRNAs(Ser) by seryl-tRNA synthetase in mammalian mitochondria., Chimnaronk S, Gravers Jeppesen M, Suzuki T, Nyborg J, Watanabe K, EMBO J. 2005 Oct 5;24(19):3369-79. Epub 2005 Sep 15. PMID:16163389

Characterization and tRNA recognition of mammalian mitochondrial seryl-tRNA synthetase., Yokogawa T, Shimada N, Takeuchi N, Benkowski L, Suzuki T, Omori A, Ueda T, Nishikawa K, Spremulli LL, Watanabe K, J Biol Chem. 2000 Jun 30;275(26):19913-20. PMID:10764807

Dual mode recognition of two isoacceptor tRNAs by mammalian mitochondrial seryl-tRNA synthetase., Shimada N, Suzuki T, Watanabe K, J Biol Chem. 2001 Dec 14;276(50):46770-8. Epub 2001 Sep 27. PMID:11577083

Crystallization and preliminary X-ray diffraction study of mammalian mitochondrial seryl-tRNA synthetase., Chimnaronk S, Jeppesen MG, Shimada N, Suzuki T, Nyborg J, Watanabe K, Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1319-22. Epub 2004, Jun 22. PMID:15213404

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