1gmn
From Proteopedia
(New page: 200px<br /> <applet load="1gmn" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gmn, resolution 2.30Å" /> '''CRYSTAL STRUCTURES ...) |
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==About this Structure== | ==About this Structure== | ||
| - | 1GMN is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with EPE as [[http://en.wikipedia.org/wiki/ligand ligand]]. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GMN OCA]]. | + | 1GMN is a [[http://en.wikipedia.org/wiki/Single_protein Single protein]] structure of sequence from [[http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]] with EPE as [[http://en.wikipedia.org/wiki/ligand ligand]]. Structure known Active Site: AC1. Full crystallographic information is available from [[http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1GMN OCA]]. |
==Reference== | ==Reference== | ||
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[[Category: hormone/growth factor]] | [[Category: hormone/growth factor]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Oct 30 12:01:43 2007'' |
Revision as of 09:57, 30 October 2007
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CRYSTAL STRUCTURES OF NK1-HEPARIN COMPLEXES REVEAL THE BASIS FOR NK1 ACTIVITY AND ENABLE ENGINEERING OF POTENT AGONISTS OF THE MET RECEPTOR
Overview
NK1 is a splice variant of the polypeptide growth factor HGF/SF, which, consists of the N-terminal (N) and first kringle (K) domain and requires, heparan sulfate or soluble heparin for activity. We describe two X-ray, crystal structures of NK1-heparin complexes that define a heparin-binding, site in the N domain, in which a major role is played by R73, with further, contributions from main chain atoms of T61, K63 and G79 and the side, chains of K60, T61, R76, K62 and K58. Mutagenesis experiments demonstrate, that heparin binding to this site is essential for dimerization in, solution and biological activity of NK1. Heparin also comes into contact, with a patch of positively charged residues (K132, R134, K170 and R181) in, the K domain. Mutation of these residues yields NK1 variants with, ... [(full description)]
About this Structure
1GMN is a [Single protein] structure of sequence from [Homo sapiens] with EPE as [ligand]. Structure known Active Site: AC1. Full crystallographic information is available from [OCA].
Reference
Crystal structures of NK1-heparin complexes reveal the basis for NK1 activity and enable engineering of potent agonists of the MET receptor., Lietha D, Chirgadze DY, Mulloy B, Blundell TL, Gherardi E, EMBO J. 2001 Oct 15;20(20):5543-55. PMID:11597998
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