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1wnd

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{{STRUCTURE_1wnd| PDB=1wnd | SCENE= }}
{{STRUCTURE_1wnd| PDB=1wnd | SCENE= }}
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'''Escherichia coli YdcW gene product is a medium-chain aldehyde dehydrogenase as determined by kinetics and crystal stucture'''
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===Escherichia coli YdcW gene product is a medium-chain aldehyde dehydrogenase as determined by kinetics and crystal stucture===
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==Overview==
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In the context of a medium-scaled structural genomics program aiming at solving the structures of as many as possible bacterial unknown open reading frame products from Escherichia coli (Y prefix), we have solved the structure of YdcW at 2.1A resolution, using molecular replacement. According to its sequence identity, YdcW has been classified into the betaine aldehyde dehydrogenases family (EC 1.2.1.8), catalysing the oxidation of betaine aldehyde into glycine betaine. The structure of YdcW resembles that of other aldehyde dehydrogenases: it is tetrameric and binds a NADH molecule in each monomer. The NADH molecules, bound in the active site by soaking, are revealed to be in the "hydrolysis position". Activities experiments demonstrate that YdcW is more active on medium-chains aldehyde than on betaine aldehyde. However, soaking of betaine into YdcW crystals revealed its presence in one of the subunits, in two positions, a putative resting position and a hydride transfer ready position. Analysis of kinetics data and of the active site shape suggest an optimum binding of n-alkyl aldehydes up to seven to eight carbon atoms, possibly followed by a bulky cyclic or aromatic group.
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(as it appears on PubMed at http://www.pubmed.gov), where 15381418 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15381418}}
==About this Structure==
==About this Structure==
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[[Category: Kinetic]]
[[Category: Kinetic]]
[[Category: Nadh]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:54:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 19:59:41 2008''

Revision as of 16:59, 27 July 2008

Image:1wnd.png

Template:STRUCTURE 1wnd

Escherichia coli YdcW gene product is a medium-chain aldehyde dehydrogenase as determined by kinetics and crystal stucture

Template:ABSTRACT PUBMED 15381418

About this Structure

1WND is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure and kinetics identify Escherichia coli YdcW gene product as a medium-chain aldehyde dehydrogenase., Gruez A, Roig-Zamboni V, Grisel S, Salomoni A, Valencia C, Campanacci V, Tegoni M, Cambillau C, J Mol Biol. 2004 Oct 8;343(1):29-41. PMID:15381418

Page seeded by OCA on Sun Jul 27 19:59:41 2008

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