1woc

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[[Image:1woc.gif|left|200px]]
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{{STRUCTURE_1woc| PDB=1woc | SCENE= }}
{{STRUCTURE_1woc| PDB=1woc | SCENE= }}
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'''Crystal structure of PriB'''
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===Crystal structure of PriB===
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==Overview==
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PriB is not only an essential protein necessary for the replication restart on the collapsed and disintegrated replication fork, but also an important protein for assembling of primosome onto PhiX174 genomic DNA during replication initiation. Here we report a 2.0-A-resolution X-ray structure of a biologically functional form of PriB from Escherichia coli. The crystal structure revealed that despite a low level of primary sequence identity, the PriB monomer, as well as the dimeric form, are structurally identical to the N-terminal DNA-binding domain of the single-stranded DNA-binding protein (SSB) from Escherichia coli, which possesses an oligonucleotides-binding-fold. The oligonucleotide-PriB complex model based on the oligonucleotides-SSB complex structure suggested that PriB had a DNA-binding pocket conserved in SSB from Escherichia coli and might bind to single-stranded DNA in the manner of SSB. Furthermore, surface plasmon resonance analysis and fluorescence measurements demonstrated that PriB binds single-stranded DNA with high affinity, by involving tryptophan residue. The significance of these results with respect to the functional role of PriB in the assembly of primosome is discussed.
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(as it appears on PubMed at http://www.pubmed.gov), where 15607735 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15607735}}
==About this Structure==
==About this Structure==
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[[Category: Ueda, T.]]
[[Category: Ueda, T.]]
[[Category: Oligonucleotide binding fold]]
[[Category: Oligonucleotide binding fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:56:35 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:45:34 2008''

Revision as of 23:45, 28 July 2008

Image:1woc.png

Template:STRUCTURE 1woc

Crystal structure of PriB

Template:ABSTRACT PUBMED 15607735

About this Structure

1WOC is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure of a biologically functional form of PriB from Escherichia coli reveals a potential single-stranded DNA-binding site., Shioi S, Ose T, Maenaka K, Shiroishi M, Abe Y, Kohda D, Katayama T, Ueda T, Biochem Biophys Res Commun. 2005 Jan 28;326(4):766-76. PMID:15607735

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