1wpt

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[[Image:1wpt.gif|left|200px]]
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{{STRUCTURE_1wpt| PDB=1wpt | SCENE= }}
{{STRUCTURE_1wpt| PDB=1wpt | SCENE= }}
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'''Crystal Structure of HutP, an RNA binding anti-termination protein'''
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===Crystal Structure of HutP, an RNA binding anti-termination protein===
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==Overview==
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HutP is an RNA-binding protein that regulates the expression of the histidine utilization (hut) operon in Bacillus subtilis, by binding to cis-acting regulatory sequences on hut mRNA. It requires L-histidine and an Mg2+ ion for binding to the specific sequence within the hut mRNA. In the present study, we show that several divalent cations can mediate the HutP-RNA interactions. The best divalent cations were Mn2+, Zn2+ and Cd2+, followed by Mg2+, Co2+ and Ni2+, while Cu2+, Yb2+ and Hg2+ were ineffective. In the HutP-RNA interactions, divalent cations cannot be replaced by monovalent cations, suggesting that a divalent metal ion is required for mediating the protein-RNA interactions. To clarify their importance, we have crystallized HutP in the presence of three different metal ions (Mg2+, Mn2+ and Ba2+), which revealed the importance of the metal ion binding site. Furthermore, these analyses clearly demonstrated how the metal ions cause the structural rearrangements that are required for the hut mRNA recognition.
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The line below this paragraph, {{ABSTRACT_PUBMED_16192572}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 16192572 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16192572}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Characterization of the metal ion binding site in the anti-terminator protein, HutP, of Bacillus subtilis., Kumarevel T, Mizuno H, Kumar PK, Nucleic Acids Res. 2005 Sep 28;33(17):5494-502. Print 2005. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16192572 16192572]
Characterization of the metal ion binding site in the anti-terminator protein, HutP, of Bacillus subtilis., Kumarevel T, Mizuno H, Kumar PK, Nucleic Acids Res. 2005 Sep 28;33(17):5494-502. Print 2005. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16192572 16192572]
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Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15242603 15242603]
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Identification of important chemical groups of the hut mRNA for HutP interactions that regulate the hut operon in Bacillus subtilis., Kumarevel TS, Gopinath SC, Nishikawa S, Mizuno H, Kumar PK, Nucleic Acids Res. 2004 Jul 25;32(13):3904-12. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15273277 15273277]
[[Category: Bacillus subtilis]]
[[Category: Bacillus subtilis]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Rna binding]]
[[Category: Rna binding]]
[[Category: Transcription regulation]]
[[Category: Transcription regulation]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 13:59:30 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 16:27:39 2008''

Revision as of 13:27, 28 July 2008

Image:1wpt.png

Template:STRUCTURE 1wpt

Crystal Structure of HutP, an RNA binding anti-termination protein

Template:ABSTRACT PUBMED 16192572

About this Structure

1WPT is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Characterization of the metal ion binding site in the anti-terminator protein, HutP, of Bacillus subtilis., Kumarevel T, Mizuno H, Kumar PK, Nucleic Acids Res. 2005 Sep 28;33(17):5494-502. Print 2005. PMID:16192572

Crystal structure of activated HutP; an RNA binding protein that regulates transcription of the hut operon in Bacillus subtilis., Kumarevel T, Fujimoto Z, Karthe P, Oda M, Mizuno H, Kumar PK, Structure. 2004 Jul;12(7):1269-80. PMID:15242603

Identification of important chemical groups of the hut mRNA for HutP interactions that regulate the hut operon in Bacillus subtilis., Kumarevel TS, Gopinath SC, Nishikawa S, Mizuno H, Kumar PK, Nucleic Acids Res. 2004 Jul 25;32(13):3904-12. Print 2004. PMID:15273277

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