1zy7
From Proteopedia
(New page: 200px<br /> <applet load="1zy7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zy7, resolution 1.70Å" /> '''Crystal structure o...) |
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| - | [[Image:1zy7.gif|left|200px]]<br /> | + | [[Image:1zy7.gif|left|200px]]<br /><applet load="1zy7" size="350" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1zy7" size=" | + | |
caption="1zy7, resolution 1.70Å" /> | caption="1zy7, resolution 1.70Å" /> | ||
'''Crystal structure of the catalytic domain of an adenosine deaminase that acts on RNA (hADAR2) bound to inositol hexakisphosphate (IHP)'''<br /> | '''Crystal structure of the catalytic domain of an adenosine deaminase that acts on RNA (hADAR2) bound to inositol hexakisphosphate (IHP)'''<br /> | ||
==Overview== | ==Overview== | ||
| - | We report the crystal structure of the catalytic domain of human ADAR2, an | + | We report the crystal structure of the catalytic domain of human ADAR2, an RNA editing enzyme, at 1.7 angstrom resolution. The structure reveals a zinc ion in the active site and suggests how the substrate adenosine is recognized. Unexpectedly, inositol hexakisphosphate (IP6) is buried within the enzyme core, contributing to the protein fold. Although there are no reports that adenosine deaminases that act on RNA (ADARs) require a cofactor, we show that IP6 is required for activity. Amino acids that coordinate IP6 in the crystal structure are conserved in some adenosine deaminases that act on transfer RNA (tRNA) (ADATs), related enzymes that edit tRNA. Indeed, IP6 is also essential for in vivo and in vitro deamination of adenosine 37 of tRNAala by ADAT1. |
==About this Structure== | ==About this Structure== | ||
| - | 1ZY7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with ZN, SO4 and IHP as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http:// | + | 1ZY7 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] with <scene name='pdbligand=ZN:'>ZN</scene>, <scene name='pdbligand=SO4:'>SO4</scene> and <scene name='pdbligand=IHP:'>IHP</scene> as [http://en.wikipedia.org/wiki/ligands ligands]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1ZY7 OCA]. |
==Reference== | ==Reference== | ||
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[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Single protein]] | [[Category: Single protein]] | ||
| - | [[Category: Bass, B | + | [[Category: Bass, B L.]] |
| - | [[Category: Hill, C | + | [[Category: Hill, C P.]] |
| - | [[Category: Lingam, A | + | [[Category: Lingam, A T.]] |
| - | [[Category: Macbeth, M | + | [[Category: Macbeth, M R.]] |
| - | [[Category: Schubert, H | + | [[Category: Schubert, H L.]] |
| - | [[Category: Vandemark, A | + | [[Category: Vandemark, A P.]] |
[[Category: IHP]] | [[Category: IHP]] | ||
[[Category: SO4]] | [[Category: SO4]] | ||
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[[Category: zinc coordination]] | [[Category: zinc coordination]] | ||
| - | ''Page seeded by [http:// | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Feb 21 16:20:15 2008'' |
Revision as of 14:20, 21 February 2008
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Crystal structure of the catalytic domain of an adenosine deaminase that acts on RNA (hADAR2) bound to inositol hexakisphosphate (IHP)
Overview
We report the crystal structure of the catalytic domain of human ADAR2, an RNA editing enzyme, at 1.7 angstrom resolution. The structure reveals a zinc ion in the active site and suggests how the substrate adenosine is recognized. Unexpectedly, inositol hexakisphosphate (IP6) is buried within the enzyme core, contributing to the protein fold. Although there are no reports that adenosine deaminases that act on RNA (ADARs) require a cofactor, we show that IP6 is required for activity. Amino acids that coordinate IP6 in the crystal structure are conserved in some adenosine deaminases that act on transfer RNA (tRNA) (ADATs), related enzymes that edit tRNA. Indeed, IP6 is also essential for in vivo and in vitro deamination of adenosine 37 of tRNAala by ADAT1.
About this Structure
1ZY7 is a Single protein structure of sequence from Homo sapiens with , and as ligands. Full crystallographic information is available from OCA.
Reference
Inositol hexakisphosphate is bound in the ADAR2 core and required for RNA editing., Macbeth MR, Schubert HL, Vandemark AP, Lingam AT, Hill CP, Bass BL, Science. 2005 Sep 2;309(5740):1534-9. PMID:16141067
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