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1ws5

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{{STRUCTURE_1ws5| PDB=1ws5 | SCENE= }}
{{STRUCTURE_1ws5| PDB=1ws5 | SCENE= }}
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'''Crystal structure of Jacalin-Me-alpha-Mannose complex: Promiscuity vs Specificity'''
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===Crystal structure of Jacalin-Me-alpha-Mannose complex: Promiscuity vs Specificity===
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==Overview==
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Jacalin, a tetrameric lectin, is one of the two lectins present in jackfruit (Artocarpus integrifolia) seeds. Its crystal structure revealed, for the first time, the occurrence of the beta-prism I fold in lectins. The structure led to the elucidation of the crucial role of a new N terminus generated by post-translational proteolysis for the lectin's specificity for galactose. Subsequent X-ray studies on other carbohydrate complexes showed that the extended binding site of jacalin consisted of, in addition to the primary binding site, a hydrophobic secondary site A composed of aromatic residues and a secondary site B involved mainly in water-bridges. A recent investigation involving surface plasmon resonance and the X-ray analysis of a methyl-alpha-mannose complex, had led to a suggestion of promiscuity in the lectin's sugar specificity. To explore this suggestion further, detailed isothermal titration calorimetric studies on the interaction of galactose (Gal), mannose (Man), glucose (Glc), Me-alpha-Gal, Me-alpha-Man, Me-alpha-Glc and other mono- and oligosaccharides of biological relevance and crystallographic studies on the jacalin-Me-alpha-Glc complex and a new form of the jacalin-Me-alpha-Man complex, have been carried out. The binding affinity of Me-alpha-Man is 20 times weaker than that of Me-alpha-Gal. The corresponding number is 27, when the binding affinities of Gal and Me-alpha-Gal, and those of Man and Me-alpha-Man are compared. Glucose (Glc) shows no measurable binding, while the binding affinity of Me-alpha-Glc is slightly less than that of Me-alpha-Man. The available crystal structures of jacalin-sugar complexes provide a convincing explanation for the energetics of binding in terms of interactions at the primary binding site and secondary site A. The other sugars used in calorimetric studies show no detectable binding to jacalin. These results and other available evidence suggest that jacalin is specific to O-glycans and its affinity to N-glycans is extremely weak or non-existent and therefore of limited value in processes involving biological recognition.
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(as it appears on PubMed at http://www.pubmed.gov), where 15733927 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15733927}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural basis for the energetics of jacalin-sugar interactions: promiscuity versus specificity., Arockia Jeyaprakash A, Jayashree G, Mahanta SK, Swaminathan CP, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2005 Mar 18;347(1):181-8. Epub 2005 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15733927 15733927]
Structural basis for the energetics of jacalin-sugar interactions: promiscuity versus specificity., Arockia Jeyaprakash A, Jayashree G, Mahanta SK, Swaminathan CP, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2005 Mar 18;347(1):181-8. Epub 2005 Jan 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15733927 15733927]
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A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold., Sankaranarayanan R, Sekar K, Banerjee R, Sharma V, Surolia A, Vijayan M, Nat Struct Biol. 1996 Jul;3(7):596-603. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8673603 8673603]
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Crystal structure of the jacalin-T-antigen complex and a comparative study of lectin-T-antigen complexes., Jeyaprakash AA, Geetha Rani P, Banuprakash Reddy G, Banumathi S, Betzel C, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2002 Aug 23;321(4):637-45. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12206779 12206779]
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Structural basis of the carbohydrate specificities of jacalin: an X-ray and modeling study., Jeyaprakash AA, Katiyar S, Swaminathan CP, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2003 Sep 5;332(1):217-28. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12946359 12946359]
[[Category: Artocarpus integer]]
[[Category: Artocarpus integer]]
[[Category: Protein complex]]
[[Category: Protein complex]]
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[[Category: Laectin]]
[[Category: Laectin]]
[[Category: Post translational proteolysis]]
[[Category: Post translational proteolysis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:04:16 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 07:42:40 2008''

Revision as of 04:42, 29 July 2008

Image:1ws5.png

Template:STRUCTURE 1ws5

Crystal structure of Jacalin-Me-alpha-Mannose complex: Promiscuity vs Specificity

Template:ABSTRACT PUBMED 15733927

About this Structure

1WS5 is a Protein complex structure of sequences from Artocarpus integer. Full crystallographic information is available from OCA.

Reference

Structural basis for the energetics of jacalin-sugar interactions: promiscuity versus specificity., Arockia Jeyaprakash A, Jayashree G, Mahanta SK, Swaminathan CP, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2005 Mar 18;347(1):181-8. Epub 2005 Jan 18. PMID:15733927

A novel mode of carbohydrate recognition in jacalin, a Moraceae plant lectin with a beta-prism fold., Sankaranarayanan R, Sekar K, Banerjee R, Sharma V, Surolia A, Vijayan M, Nat Struct Biol. 1996 Jul;3(7):596-603. PMID:8673603

Crystal structure of the jacalin-T-antigen complex and a comparative study of lectin-T-antigen complexes., Jeyaprakash AA, Geetha Rani P, Banuprakash Reddy G, Banumathi S, Betzel C, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2002 Aug 23;321(4):637-45. PMID:12206779

Structural basis of the carbohydrate specificities of jacalin: an X-ray and modeling study., Jeyaprakash AA, Katiyar S, Swaminathan CP, Sekar K, Surolia A, Vijayan M, J Mol Biol. 2003 Sep 5;332(1):217-28. PMID:12946359

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