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1ws8

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{{STRUCTURE_1ws8| PDB=1ws8 | SCENE= }}
{{STRUCTURE_1ws8| PDB=1ws8 | SCENE= }}
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'''Crystal Structure of Mavicyanin from Cucurbita pepo medullosa (Zucchini)'''
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===Crystal Structure of Mavicyanin from Cucurbita pepo medullosa (Zucchini)===
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==Overview==
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Mavicyanin, a glycosylated protein isolated from Cucurbita pepo medullosa (zucchini), is a member of the phytocyanin subfamily containing one polypeptide chain of 109 amino residues and an unusual type-I Cu site in which the copper ligands are His45, Cys86, His91, and Gln96. The crystal structures of oxidized and reduced mavicyanin were determined at 1.6 and 1.9 A resolution, respectively. Mavicyanin has a core structure of seven polypeptide beta-strands arranged as a beta-sandwich organized into two beta-sheets, and the structure considerably resembles that of stellacyanin from cucumber (CST) or cucumber basic protein (CBP). A flexible region was not observed on superimpositioning of the oxidized and reduced mavicyanin structures. However, the Cu(II)-epsilon-O-Gln96 bond length was extended by 0.47 A, and the Thr15 residue was rotated by 60.0 degrees and O-gamma1-Thr15 moved from a distance of 4.78 to 2.58 A from the ligand Gln96 forming a new hydrogen bond between O-gamma1-Thr15 and epsilon-O-Gln96 upon reduction. The reorganization of copper coordination geometry of mavicyanin upon reduction arouses reduction potential decreased above pH 8 [Battistuzzi et al. (2001) J. Inorg. Biochem. 83, 223-227]. The rotation of Thr15 and the hydrogen bonding with the ligand Gln96 may constitute structural evidence of the decrease in the reduction potential at high pH.
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{{ABSTRACT_PUBMED_15858169}}
==About this Structure==
==About this Structure==
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[[Category: Oxidized form]]
[[Category: Oxidized form]]
[[Category: Phytocyanin]]
[[Category: Phytocyanin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 02:39:18 2008''

Revision as of 23:39, 28 July 2008

Image:1ws8.png

Template:STRUCTURE 1ws8

Crystal Structure of Mavicyanin from Cucurbita pepo medullosa (Zucchini)

Template:ABSTRACT PUBMED 15858169

About this Structure

1WS8 is a Single protein structure of sequence from Cucurbita pepo. Full crystallographic information is available from OCA.

Reference

Structural reorganization of the copper binding site involving Thr15 of mavicyanin from Cucurbita pepo medullosa (zucchini) upon reduction., Xie Y, Inoue T, Miyamoto Y, Matsumura H, Kataoka K, Yamaguchi K, Nojini M, Suzuki S, Kai Y, J Biochem. 2005 Apr;137(4):455-61. PMID:15858169

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