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1wsv

From Proteopedia

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[[Image:1wsv.gif|left|200px]]
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{{STRUCTURE_1wsv| PDB=1wsv | SCENE= }}
{{STRUCTURE_1wsv| PDB=1wsv | SCENE= }}
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'''Crystal Structure of Human T-protein of Glycine Cleavage System'''
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===Crystal Structure of Human T-protein of Glycine Cleavage System===
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==Overview==
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T-protein, a component of the glycine cleavage system, catalyzes the formation of ammonia and 5,10-methylenetetrahydrofolate from the aminomethyl moiety of glycine attached to the lipoate cofactor of H-protein. Several mutations in the human T-protein gene cause non-ketotic hyperglycinemia. To gain insights into the effect of disease-causing mutations and the catalytic mechanism at the molecular level, crystal structures of human T-protein in free form and that bound to 5-methyltetrahydrofolate (5-CH3-H4folate) have been determined at 2.0 A and 2.6 A resolution, respectively. The overall structure consists of three domains arranged in a cloverleaf-like structure with the central cavity, where 5-CH3-H4folate is bound in a kinked shape with the pteridine group deeply buried into the hydrophobic pocket and the glutamyl group pointed to the C-terminal side surface. Most of the disease-related residues cluster around the cavity, forming extensive hydrogen bonding networks. These hydrogen bonding networks are employed in holding not only the folate-binding space but also the positions and the orientations of alpha-helix G and the following loop in the middle region, which seems to play a pivotal role in the T-protein catalysis. Structural and mutational analyses demonstrated that Arg292 interacts through water molecules with the folate polyglutamate tail, and that the invariant Asp101, located close to the N10 group of 5-CH3-H4folate, might play a key role in the initiation of the catalysis by increasing the nucleophilic character of the N10 atom of the folate substrate for the nucleophilic attack on the aminomethyl lipoate intermediate. A clever mechanism of recruiting the aminomethyl lipoate arm to the reaction site seems to function as a way of avoiding the release of toxic formaldehyde.
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(as it appears on PubMed at http://www.pubmed.gov), where 16051266 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16051266}}
==About this Structure==
==About this Structure==
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[[Category: Aminomethyl transferase]]
[[Category: Aminomethyl transferase]]
[[Category: Glycine-cleavage sytem]]
[[Category: Glycine-cleavage sytem]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:05:38 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 21:41:03 2008''

Revision as of 18:41, 28 July 2008

Image:1wsv.png

Template:STRUCTURE 1wsv

Crystal Structure of Human T-protein of Glycine Cleavage System

Template:ABSTRACT PUBMED 16051266

About this Structure

1WSV is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Crystal structure of human T-protein of glycine cleavage system at 2.0 A resolution and its implication for understanding non-ketotic hyperglycinemia., Okamura-Ikeda K, Hosaka H, Yoshimura M, Yamashita E, Toma S, Nakagawa A, Fujiwara K, Motokawa Y, Taniguchi H, J Mol Biol. 2005 Sep 2;351(5):1146-59. PMID:16051266

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