1wu4

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[[Image:1wu4.gif|left|200px]]
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{{STRUCTURE_1wu4| PDB=1wu4 | SCENE= }}
{{STRUCTURE_1wu4| PDB=1wu4 | SCENE= }}
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'''Crystal structure of reducing-end-xylose releasing exo-oligoxylanase'''
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===Crystal structure of reducing-end-xylose releasing exo-oligoxylanase===
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==Overview==
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Reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125 (Rex) hydrolyzes xylooligosaccharides whose degree of polymerization is greater than or equal to 3, releasing the xylose unit at the reducing end. It is a unique exo-type glycoside hydrolase that recognizes the xylose unit at the reducing end in a very strict manner, even discriminating the beta-anomeric hydroxyl configuration from the alpha-anomer or 1-deoxyxylose. We have determined the crystal structures of Rex in unliganded and complex forms at 1.35-2.20-A resolution and revealed the structural aspects of its three subsites ranging from -2 to +1. The structure of Rex was compared with those of endo-type enzymes in glycoside hydrolase subfamily 8a (GH-8a). The catalytic machinery of Rex is basically conserved with other GH-8a enzymes. However, subsite +2 is blocked by a barrier formed by a kink in the loop before helix alpha10. His-319 in this loop forms a direct hydrogen bond with the beta-hydroxyl of xylose at subsite +1, contributing to the specific recognition of anomers at the reducing end.
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The line below this paragraph, {{ABSTRACT_PUBMED_15718242}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15718242 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15718242}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125., Fushinobu S, Hidaka M, Honda Y, Wakagi T, Shoun H, Kitaoka M, J Biol Chem. 2005 Apr 29;280(17):17180-6. Epub 2005 Feb 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15718242 15718242]
Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125., Fushinobu S, Hidaka M, Honda Y, Wakagi T, Shoun H, Kitaoka M, J Biol Chem. 2005 Apr 29;280(17):17180-6. Epub 2005 Feb 17. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15718242 15718242]
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A family 8 glycoside hydrolase from Bacillus halodurans C-125 (BH2105) is a reducing end xylose-releasing exo-oligoxylanase., Honda Y, Kitaoka M, J Biol Chem. 2004 Dec 31;279(53):55097-103. Epub 2004 Oct 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15491996 15491996]
[[Category: Bacteria]]
[[Category: Bacteria]]
[[Category: Oligosaccharide reducing-end xylanase]]
[[Category: Oligosaccharide reducing-end xylanase]]
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[[Category: Wakagi, T.]]
[[Category: Wakagi, T.]]
[[Category: Glycoside hydrolase family 8]]
[[Category: Glycoside hydrolase family 8]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:08:18 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:47:10 2008''

Revision as of 01:47, 28 July 2008

Image:1wu4.png

Template:STRUCTURE 1wu4

Crystal structure of reducing-end-xylose releasing exo-oligoxylanase

Template:ABSTRACT PUBMED 15718242

About this Structure

1WU4 is a Single protein structure of sequence from Bacteria. Full crystallographic information is available from OCA.

Reference

Structural basis for the specificity of the reducing end xylose-releasing exo-oligoxylanase from Bacillus halodurans C-125., Fushinobu S, Hidaka M, Honda Y, Wakagi T, Shoun H, Kitaoka M, J Biol Chem. 2005 Apr 29;280(17):17180-6. Epub 2005 Feb 17. PMID:15718242

A family 8 glycoside hydrolase from Bacillus halodurans C-125 (BH2105) is a reducing end xylose-releasing exo-oligoxylanase., Honda Y, Kitaoka M, J Biol Chem. 2004 Dec 31;279(53):55097-103. Epub 2004 Oct 18. PMID:15491996

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