1wvl

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[[Image:1wvl.gif|left|200px]]
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{{STRUCTURE_1wvl| PDB=1wvl | SCENE= }}
{{STRUCTURE_1wvl| PDB=1wvl | SCENE= }}
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'''Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer'''
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===Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer===
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==Overview==
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The protein Sac7d belongs to a class of small chromosomal proteins from the hyperthermophilic archaeon Sulfolobus acidocaldarius. Sac7d is extremely stable to heat, acid, and chemical agents. This protein is a monomer and it binds DNA without any particular sequence preference, while inducing a sharp kink in the DNA. By appending a leucine-zipper-like helical peptide derived from the yeast transcriptional activator GCN4 to the C-terminal end of Sac7d, the modified monomers (denoted S7dLZ) are expected to interact with each other via hydrophobic force to form a parallel dimer. The recombinant S7dLZ was expressed in Escherichia coli and purified by heating and ion-exchange chromatography. The formation of dimer was detected by gel-filtration chromatography and chemical cross-link. The results of surface plasmon resonance and circular dichroism experiments showed that the DNA-binding capacity was retained. Furthermore, X-ray diffraction analysis of single crystals of S7dLZ in complex with DNA decamer CCTATATAGG showed that the leucine-zipper segments of S7dLZ were associated into an antiparallel four-helix bundle. There are two DNA fragments bound to each S7dLZ tetramer in the crystal. This model works as a successful template that endows protein a new function without losing original properties.
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{{ABSTRACT_PUBMED_16028219}}
==About this Structure==
==About this Structure==
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[[Category: Thermophile]]
[[Category: Thermophile]]
[[Category: X-ray crystallography]]
[[Category: X-ray crystallography]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:11:43 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 13:28:45 2008''

Revision as of 10:28, 29 July 2008

Image:1wvl.png

Template:STRUCTURE 1wvl

Crystal Structure of Multimeric DNA-binding Protein Sac7d-GCN4 with DNA decamer

Template:ABSTRACT PUBMED 16028219

About this Structure

1WVL is a Single protein structure of sequence from Sulfolobus acidocaldarius. Full crystallographic information is available from OCA.

Reference

Design and characterization of a multimeric DNA binding protein using Sac7d and GCN4 as templates., Wu SW, Ko TP, Chou CC, Wang AH, Proteins. 2005 Sep 1;60(4):617-28. PMID:16028219

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