TRPC1 TRPC4 8WPL BI3323 Aug2025

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== Structural highlights ==1. The channel, when TRPC1 is incorporated, loses the 4-fold symmetry with one TRPC1 and three TRPC4. This change in arrangement breaks the symmetry of the pore and creates a different, asymmetric ion-conduction pathway.{{Scene|PDB=8WPL|Scene=Asymmetric_Tetramer|Text=asymmetric 1:3 stoichiometry|Commands=select all; color chain; zoom; orient center}} <ref>Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. ''Nature Structural & Molecular Biology'', 32(2):326–338. DOI: 10.1038/s41594-024-01408-1</ref>
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== Structural highlights ==1. The channel, when TRPC1 is incorporated, loses the 4-fold symmetry with one TRPC1 and three TRPC4. This change in arrangement breaks the symmetry of the pore and creates a different, asymmetric ion-conduction pathway.{{Scene|PDB=8WPL|Scene=Asymmetric_Tetramer|Text=asymmetric ion-conduction pathway|Commands=select all; color chain; zoom; orient center}} <ref>Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. ''Nature Structural & Molecular Biology'', 32(2):326–338. DOI: 10.1038/s41594-024-01408-1</ref>
2. The selectivity filter loop(two maino acids longer than the corresponding loop in TRPC4) causes the loop to protrude further into the pore. L601 from TRPC1 is the key residue responsible for this, as it physically projects into the ion pathway, narrowing the pore radius. These change the channels' preference for monovalent channels.{{Scene|PDB=8WPL|Scene=L601_Select_Filter|Text=L601 residue in the selectivity filter|Commands=select all; spacefill off; backbone off; cartoon; color cartoon chain; select :601.D; wireframe 0.2; color magenta, :601.D; zoom; orient center}}
2. The selectivity filter loop(two maino acids longer than the corresponding loop in TRPC4) causes the loop to protrude further into the pore. L601 from TRPC1 is the key residue responsible for this, as it physically projects into the ion pathway, narrowing the pore radius. These change the channels' preference for monovalent channels.{{Scene|PDB=8WPL|Scene=L601_Select_Filter|Text=L601 residue in the selectivity filter|Commands=select all; spacefill off; backbone off; cartoon; color cartoon chain; select :601.D; wireframe 0.2; color magenta, :601.D; zoom; orient center}}
<ref>Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. ''Nature Structural & Molecular Biology'', 32(2):326–338. DOI: 10.1038/s41594-024-01408-1</ref>
<ref>Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. ''Nature Structural & Molecular Biology'', 32(2):326–338. DOI: 10.1038/s41594-024-01408-1</ref>

Revision as of 14:06, 30 November 2025

Overview of the TRPC1/TRPC4 Channel

Cryo-EM structure of the heteromeric human TRPC1/TRPC4 channel (PDB: 8WPL)

Drag the structure with the mouse to rotate

References

  1. ↑ Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nature Structural & Molecular Biology, 32(2):326–338. DOI: 10.1038/s41594-024-01408-1
  2. ↑ Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nature Structural & Molecular Biology, 32(2):326–338. DOI: 10.1038/s41594-024-01408-1
  3. ↑ Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nature Structural & Molecular Biology, 32(2):326–338. DOI: 10.1038/s41594-024-01408-1
  4. ↑ Pani, B., Cornatzer, E. et al. (2006). Up-Regulation of Transient Receptor Potential Canonical 1 (TRPC1) following Sarco(endo)plasmic Reticulum Ca²⁺ ATPase 2 Gene Silencing Promotes Cell Survival: A Potential Role for TRPC1 in Darier's Disease. Molecular Biology of the Cell, 17(10):4446–4458.
  5. ↑ Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nature Structural & Molecular Biology, 32(2):326–338. DOI: 10.1038/s41594-024-01408-1
  6. ↑ Jeon, J., Moore, T. I., Sob, I. et al. (2025). TRPC4 regulates limbic behavior and neuronal development by stabilizing dendrite branches through actomyosin-driven integrin activation. PNAS, 122(33):e2511037ca122.
  7. ↑ Pani, B., Cornatzer, E. et al. (2006). Up-Regulation of Transient Receptor Potential Canonical 1 (TRPC1) following Sarco(endo)plasmic Reticulum Ca²⁺ ATPase 2 Gene Silencing Promotes Cell Survival: A Potential Role for TRPC1 in Darier's Disease. Molecular Biology of the Cell, 17(10):4446–4458.
  8. ↑ Jeon, J., Moore, T. I., Sob, I. et al. (2025). TRPC4 regulates limbic behavior and neuronal development by stabilizing dendrite branches through actomyosin-driven integrin activation. PNAS, 122(33):e2511037122.
  9. ↑ Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nature Structural & Molecular Biology, 32(2):326–338. DOI: 10.1038/s41594-024-01408-1
  10. ↑ Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nature Structural & Molecular Biology, 32(2):326–338. DOI: 10.1038/s41594-024-01408-1
  11. ↑ Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nature Structural & Molecular Biology, 32(2):326–338. DOI: 10.1038/s41594-024-01408-1
  12. ↑ Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nature Structural & Molecular Biology, 32(2):326–338. DOI: 10.1038/s41594-024-01408-1
  13. ↑ Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nature Structural & Molecular Biology, 32(2):326–338. DOI: 10.1038/s41594-024-01408-1
  14. ↑ Won, J., Kim, J., Kim, J. et al. (2025). Cryo-EM structure of the heteromeric TRPC1/TRPC4 channel. Nature Structural & Molecular Biology, 32(2):326–338. DOI: 10.1038/s41594-024-01408-1

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