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| | {{STRUCTURE_1x1h| PDB=1x1h | SCENE= }} | | {{STRUCTURE_1x1h| PDB=1x1h | SCENE= }} |
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| - | '''Crystal Structure of Xanthan Lyase (N194A)'''
| + | ===Crystal Structure of Xanthan Lyase (N194A)=== |
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| - | ==Overview==
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| - | Bacillus sp. GL1 xanthan lyase, a member of polysaccharide lyase family 8 (PL-8), acts exolytically on the side-chains of pentasaccharide-repeating polysaccharide xanthan and cleaves the glycosidic bond between glucuronic acid (GlcUA) and pyruvylated mannose (PyrMan) through a beta-elimination reaction. To clarify the enzyme reaction mechanism, i.e. its substrate recognition and catalytic reaction, we determined crystal structures of a mutant enzyme, N194A, in complexes with the product (PyrMan) and a substrate (pentasacharide) and in a ligand-free form at 1.8, 2.1, and 2.3A resolution. Based on the structures of the mutant in complexes with the product and substrate, we found that xanthan lyase recognized the PyrMan residue at subsite -1 and the GlcUA residue at +1 on the xanthan side-chain and underwent little interaction with the main chain of the polysaccharide. The structure of the mutant-substrate complex also showed that the hydroxyl group of Tyr255 was close to both the C-5 atom of the GlcUA residue and the oxygen atom of the glycosidic bond to be cleaved, suggesting that Tyr255 likely acts as a general base that extracts the proton from C-5 of the GlcUA residue and as a general acid that donates the proton to the glycosidic bond. A structural comparison of catalytic centers of PL-8 lyases indicated that the catalytic reaction mechanism is shared by all members of the family PL-8, while the substrate recognition mechanism differs.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15979090}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15979090 is the PubMed ID number. |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Alpha/alpha barrel]] | | [[Category: Alpha/alpha barrel]] |
| | [[Category: Beta sandwich]] | | [[Category: Beta sandwich]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:24:27 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:31:11 2008'' |
Revision as of 14:31, 28 July 2008
Template:STRUCTURE 1x1h
Crystal Structure of Xanthan Lyase (N194A)
Template:ABSTRACT PUBMED 15979090
About this Structure
1X1H is a Single protein structure of sequence from Bacillus sp. gl1. Full crystallographic information is available from OCA.
Reference
Crystal structure of Bacillus sp. GL1 xanthan lyase complexed with a substrate: insights into the enzyme reaction mechanism., Maruyama Y, Hashimoto W, Mikami B, Murata K, J Mol Biol. 2005 Jul 29;350(5):974-86. PMID:15979090
Page seeded by OCA on Mon Jul 28 17:31:11 2008
