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1x2i

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{{STRUCTURE_1x2i| PDB=1x2i | SCENE= }}
{{STRUCTURE_1x2i| PDB=1x2i | SCENE= }}
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'''Crystal Structure Of Archaeal Xpf/Mus81 Homolog, Hef From Pyrococcus Furiosus, Helix-hairpin-helix Domain'''
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===Crystal Structure Of Archaeal Xpf/Mus81 Homolog, Hef From Pyrococcus Furiosus, Helix-hairpin-helix Domain===
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==Overview==
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XPF/Rad1/Mus81/Hef proteins recognize and cleave branched DNA structures. XPF and Rad1 proteins cleave the 5' side of nucleotide excision repair bubble, while Mus81 and Hef cleave similar sites of the nicked Holliday junction, fork, or flap structure. These proteins all function as dimers and consist of catalytic and helix-hairpin-helix DNA binding (HhH) domains. We have determined the crystal structure of the HhH domain of Pyrococcus furiosus Hef nuclease (HefHhH), which revealed the distinct mode of protein dimerization. Our structural and biochemical analyses also showed that each of the catalytic and HhH domains binds to distinct regions within the fork-structured DNA: each HhH domain from two separate subunits asymmetrically binds to the arm region, while the catalytic domain binds near the junction center. Upon binding to DNA, Hef nuclease disrupts base pairs near the cleavage site. It is most likely that this bipartite binding mode is conserved in the XPF/Rad1/Mus81 nuclease family.
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{{ABSTRACT_PUBMED_16084390}}
==About this Structure==
==About this Structure==
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[[Category: Helix-hairpin-helix dna binding domain]]
[[Category: Helix-hairpin-helix dna binding domain]]
[[Category: Homodimer]]
[[Category: Homodimer]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:26:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 20:44:34 2008''

Revision as of 17:44, 27 July 2008

Image:1x2i.png

Template:STRUCTURE 1x2i

Crystal Structure Of Archaeal Xpf/Mus81 Homolog, Hef From Pyrococcus Furiosus, Helix-hairpin-helix Domain

Template:ABSTRACT PUBMED 16084390

About this Structure

1X2I is a Single protein structure of sequence from Pyrococcus furiosus. Full crystallographic information is available from OCA.

Reference

Structural and functional analyses of an archaeal XPF/Rad1/Mus81 nuclease: asymmetric DNA binding and cleavage mechanisms., Nishino T, Komori K, Ishino Y, Morikawa K, Structure. 2005 Aug;13(8):1183-92. PMID:16084390

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