1x2x

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{{STRUCTURE_1x2x| PDB=1x2x | SCENE= }}
{{STRUCTURE_1x2x| PDB=1x2x | SCENE= }}
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'''NMR solution structures of a DNA dodecamer containing a tandem GT mismatches using NOE and residual dipolar couplings'''
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===NMR solution structures of a DNA dodecamer containing a tandem GT mismatches using NOE and residual dipolar couplings===
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==Overview==
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We have carried out a solution study of two non-labelled self-complementary DNA dodecamers d(GACTGTACAGTC)2 and d(GACTGTGCAGTC)2 by NMR, the second sequence composed of two G-T mismatches. Structures were determined using distances extracted from NOE effects alone or using both NOE and RDC constraints, measured in three different liquid crystalline media. We ensured that our data on the influence of the mesogen on the DNA structures, and the way in which the RDCs were incorporated as constraints in the protocol refinement, were consistent. We also tested the influence of different sets of RDCs and the best means of optimizing the calculation of D(a) and R. Resolution and accuracy of the ten best energy final structures were compared. The addition of a small set of RDC constraints significantly improves the final determined structures. We took advantage of the specificity of the RDC, i.e. it contains orientational information, and explored the global shape of the DNA duplexes; it was found that the duplexes do not have a large curvature. For the G-T base pair, we observed, in this particular sequence (tandem of G-T mismatches), a new pattern of base pairing, which involved the formation of a bifurcated hydrogen bond.
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(as it appears on PubMed at http://www.pubmed.gov), where 16972306 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16972306}}
==About this Structure==
==About this Structure==
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X2X OCA].
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Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1X2X OCA].
==Reference==
==Reference==
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[[Category: Rdc]]
[[Category: Rdc]]
[[Category: Tandem gt mismatch]]
[[Category: Tandem gt mismatch]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:27:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 03:03:33 2008''

Revision as of 00:03, 28 July 2008

Image:1x2x.png

Template:STRUCTURE 1x2x

NMR solution structures of a DNA dodecamer containing a tandem GT mismatches using NOE and residual dipolar couplings

Template:ABSTRACT PUBMED 16972306

About this Structure

Full experimental information is available from OCA.

Reference

NMR assessment of the global shape of a non-labelled DNA dodecamer containing a tandem of G-T mismatches., Alvarez-Salgado F, Desvaux H, Boulard Y, Magn Reson Chem. 2006 Dec;44(12):1081-9. PMID:16972306

Page seeded by OCA on Mon Jul 28 03:03:33 2008

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