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| | {{STRUCTURE_1x7o| PDB=1x7o | SCENE= }} | | {{STRUCTURE_1x7o| PDB=1x7o | SCENE= }} |
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| - | '''Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes'''
| + | ===Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes=== |
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| - | ==Overview==
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| - | The emergence of antibiotic-resistant bacterial strains is a widespread problem in medical practice and drug design, and each case requires the elucidation of the underlying mechanism. AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of U2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide (orthosomycin) antibiotic avilamycin. The structure of AviRb with and without bound cofactor S-adenosyl-L-methionine (AdoMet) was determined, showing that it is a homodimer belonging to the SpoU family within the SPOUT class of methyltransferases. The relationships within this class were analyzed in detail and, in addition, a novel fourth SpoU sequence fingerprint is proposed. Each subunit of AviRb consists of two domains. The N-terminal domain, being related to the ribosomal proteins L30 and L7Ae, is likely to bind RNA. The C-terminal domain is related to all SPOUT methyltransferases, and is responsible for AdoMet-binding, catalysis and dimerization. The cofactor binds at the characteristic knot of the polypeptide in an unusually bent conformation. The transferred methyl group points to a broad cleft formed with the L30-type domain of the other subunit. Measurements of mutant activity revealed four important residues responsible for catalysis and allowed the modeling of a complex between AviRb and the RNA target. The model includes a specificity pocket for uracil but does not contain a base for deprotonating the 2'-O atom of U2479 on methylation. | + | The line below this paragraph, {{ABSTRACT_PUBMED_15581897}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15581897 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15581897}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Semet]] | | [[Category: Semet]] |
| | [[Category: Spou]] | | [[Category: Spou]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:40:19 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 12:21:23 2008'' |
Revision as of 09:21, 27 July 2008
Template:STRUCTURE 1x7o
Crystal structure of the SpoU Methyltransferase AviRb from Streptomyces viridochromogenes
Template:ABSTRACT PUBMED 15581897
About this Structure
1X7O is a Single protein structure of sequence from Streptomyces viridochromogenes. Full crystallographic information is available from OCA.
Reference
Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes., Mosbacher TG, Bechthold A, Schulz GE, J Mol Biol. 2005 Jan 21;345(3):535-45. PMID:15581897
Page seeded by OCA on Sun Jul 27 12:21:23 2008
