This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.


1xcb

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1xcb.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1xcb.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1xcb| PDB=1xcb | SCENE= }}
{{STRUCTURE_1xcb| PDB=1xcb | SCENE= }}
-
'''X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus'''
+
===X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus===
-
==Overview==
+
<!--
-
The redox-sensing repressor Rex regulates transcription of respiratory genes in response to the intra cellular NADH/NAD(+) redox poise. As a step toward elucidating the molecular mechanism of NADH/NAD(+) sensing, the X-ray structure of Thermus aquaticus Rex (T-Rex) bound to effector NADH has been determined at 2.9 A resolution. The fold of the C-terminal domain of T-Rex is characteristic of NAD(H)-dependent enzymes, whereas the N-terminal domain is similar to a winged helix DNA binding motif. T-Rex dimerization is primarily mediated by "domain-swapped" alpha helices. Each NADH molecule binds to the C-terminal domain near the dimer interface. In contrast to NAD(H)-dependent enzymes, the nicotinamide is deeply buried within a hydrophobic pocket that appears to preclude substrate entry. We show that T-Rex binds to the Rex operator, and NADH but not NAD(+) inhibits T-Rex/DNA binding activity. A mechanism for redox sensing by Rex family members is proposed by analogy with domain closure of NAD(H)-dependent enzymes.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15642260}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15642260 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15642260}}
==About this Structure==
==About this Structure==
Line 42: Line 46:
[[Category: Thermus aquaticus]]
[[Category: Thermus aquaticus]]
[[Category: Winged helix]]
[[Category: Winged helix]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:50:50 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 15:59:55 2008''

Revision as of 12:59, 28 July 2008

Image:1xcb.png

Template:STRUCTURE 1xcb

X-ray Structure of a Rex-Family Repressor/NADH Complex from Thermus Aquaticus

Template:ABSTRACT PUBMED 15642260

About this Structure

1XCB is a Single protein structure of sequence from Thermus aquaticus. This structure supersedes the now removed PDB entry 1r72. Full crystallographic information is available from OCA.

Reference

X-ray structure of a Rex-family repressor/NADH complex insights into the mechanism of redox sensing., Sickmier EA, Brekasis D, Paranawithana S, Bonanno JB, Paget MS, Burley SK, Kielkopf CL, Structure. 2005 Jan;13(1):43-54. PMID:15642260

Page seeded by OCA on Mon Jul 28 15:59:55 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xcb"
Personal tools