1xco

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{{STRUCTURE_1xco| PDB=1xco | SCENE= }}
{{STRUCTURE_1xco| PDB=1xco | SCENE= }}
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'''Crystal Structure of a Phosphotransacetylase from Bacillus subtilis in complex with acetylphosphate'''
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===Crystal Structure of a Phosphotransacetylase from Bacillus subtilis in complex with acetylphosphate===
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==Overview==
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Phosphotransacetylase (Pta) [EC 2.3.1.8] plays a major role in acetate metabolism by catalyzing the reversible transfer of the acetyl group between coenzyme A (CoA) and orthophosphate: CH(3)COSCoA+HPO(4)(2-)&lt;--&gt;CH(3)COOPO(3)(2-) +CoASH. In this study, we report the crystal structures of Pta from Bacillus subtilis at 2.75 A resolution and its complex with acetyl phosphate, one of its substrates, at 2.85 A resolution. In addition, the Pta activity of the enzyme has been assayed. The enzyme folds into an alpha/beta architecture with two domains separated by a prominent cleft, very similar to two other known Pta structures. The enzyme-acetyl phosphate complex structure reveals a few potential substrate binding sites. Two of them are located in the middle of the interdomain cleft: each one is surrounded by a region of strictly and highly conserved residues. High structural similarities are found with 4-hydroxythreonine-4-phosphate dehydrogenase (PdxA), and isocitrate and isopropylmalate dehydrogenases, all of which utilize NADP+ as their cofactor, which binds in the interdomain cleft. Their substrate binding sites are close to the acetyl phosphate binding sites of Pta in the cleft as well. These results suggest that the CoA is likely to bind to the interdomain cleft of Pta in a similar way as NADP+ binds to the other three enzymes.
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(as it appears on PubMed at http://www.pubmed.gov), where 16283428 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16283428}}
==About this Structure==
==About this Structure==
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[[Category: Psi]]
[[Category: Psi]]
[[Category: Structural genomic]]
[[Category: Structural genomic]]
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Revision as of 23:12, 28 July 2008

Image:1xco.png

Template:STRUCTURE 1xco

Crystal Structure of a Phosphotransacetylase from Bacillus subtilis in complex with acetylphosphate

Template:ABSTRACT PUBMED 16283428

About this Structure

1XCO is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Crystal structures of a phosphotransacetylase from Bacillus subtilis and its complex with acetyl phosphate., Xu QS, Jancarik J, Lou Y, Kuznetsova K, Yakunin AF, Yokota H, Adams P, Kim R, Kim SH, J Struct Funct Genomics. 2005 Dec;6(4):269-79. Epub 2005 Nov 9. PMID:16283428

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