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| | {{STRUCTURE_1xf2| PDB=1xf2 | SCENE= }} | | {{STRUCTURE_1xf2| PDB=1xf2 | SCENE= }} |
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| - | '''Structure of Fab DNA-1 complexed with dT3'''
| + | ===Structure of Fab DNA-1 complexed with dT3=== |
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| - | ==Overview==
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| - | Anti-DNA antibodies play important roles in the pathogenesis of autoimmune diseases. They also represent a unique and relatively unexplored class of DNA-binding protein. Here, we present a study of conformational changes induced by DNA binding to an anti-ssDNA Fab known as DNA-1. Three crystal structures are reported: a complex of DNA-1 bound to dT3, and two structures of the ligand-free Fab. One of the ligand-free structures was determined from crystals exhibiting perfect hemihedral twinning, and the details of structure determination are provided. Unexpectedly, five residues (H97-H100A) in the apex of heavy chain complementarity-determining region 3 (HCDR3) are disordered in both ligand-free structures. Ligand binding also caused a 2-4A shift of the backbone of Tyr L92 and ordering of the L92 side-chain. In contrast, these residues are highly ordered in the Fab/dT3 complex, where Tyr H100 and Tyr H100A form intimate stacking interactions with DNA bases, and L92 forms the 5' end of the binding site. The structures suggest that HCDR3 is very flexible and adopts multiple conformations in the ligand-free state. These results are discussed in terms of induced fit and pre-existing equilibrium theories of ligand binding. Our results allow new interpretations of existing thermodynamic and mutagenesis data in terms of conformational entropy and the volume of conformational space accessible to HCDR3 in the ligand-free state. In the context of autoimmune disease, plasticity of the ligand-free antibody could provide a mechanism by which anti-DNA antibodies bind diverse host ligands, and thereby contribute to pathogenicity.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_15784256}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 15784256 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_15784256}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: Fab]] | | [[Category: Fab]] |
| | [[Category: Immunoglobulin]] | | [[Category: Immunoglobulin]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 14:56:43 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 12:47:07 2008'' |
Revision as of 09:47, 29 July 2008
Template:STRUCTURE 1xf2
Structure of Fab DNA-1 complexed with dT3
Template:ABSTRACT PUBMED 15784256
About this Structure
1XF2 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Evidence for structural plasticity of heavy chain complementarity-determining region 3 in antibody-ssDNA recognition., Schuermann JP, Prewitt SP, Davies C, Deutscher SL, Tanner JJ, J Mol Biol. 2005 Apr 15;347(5):965-78. PMID:15784256
Page seeded by OCA on Tue Jul 29 12:47:07 2008
