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1xkg

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{{STRUCTURE_1xkg| PDB=1xkg | SCENE= }}
{{STRUCTURE_1xkg| PDB=1xkg | SCENE= }}
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'''Crystal structure of the major house dust mite allergen Der p 1 in its pro form at 1.61 A resolution'''
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===Crystal structure of the major house dust mite allergen Der p 1 in its pro form at 1.61 A resolution===
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==Overview==
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Allergy to house dust mite is among the most prevalent allergic diseases worldwide. Most house dust mite allergic patients react to Der p 1 from Dermatophagoides pteronyssinus, which is a cysteine protease. To avoid heterogeneity in the sample used for crystallization, a modified recombinant molecule was produced. The sequence of the proDer p 1 allergen was modified to reduce glycosylation and to abolish enzymatic activity. The resulting rproDer p 1 preparation was homogenous and stable and yielded crystals diffracting to a resolution of 1.61 A. The active site is located in a large cleft on the surface of the molecule. The 80-aa pro-peptide adopts a unique fold that interacts with the active site cleft and a substantial adjacent area on the mature region, excluding access to the cleft and the active site. Studies performed using crossed-line immunoelectrophoresis and IgE inhibition experiments indicated that several epitopes are covered by the pro-peptide and that the epitopes on the recombinant mature molecule are indistinguishable from those on the natural one. The structure confirms previous results suggesting a preference for aliphatic residues in the important P2 position in substrates. Sequence variations in related species are concentrated on the surface, which explains the existence of cross-reacting and species-specific antibodies. This study describes the first crystal structure of one of the clinically most important house dust mite allergens, the cysteine protease Der p 1.
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(as it appears on PubMed at http://www.pubmed.gov), where 16148130 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16148130}}
==About this Structure==
==About this Structure==
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[[Category: Major allergen]]
[[Category: Major allergen]]
[[Category: Pro peptide]]
[[Category: Pro peptide]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:08:40 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Jul 27 14:57:31 2008''

Revision as of 11:57, 27 July 2008

Image:1xkg.png

Template:STRUCTURE 1xkg

Crystal structure of the major house dust mite allergen Der p 1 in its pro form at 1.61 A resolution

Template:ABSTRACT PUBMED 16148130

About this Structure

1XKG is a Single protein structure of sequence from Dermatophagoides pteronyssinus. Full crystallographic information is available from OCA.

Reference

The crystal structure of recombinant proDer p 1, a major house dust mite proteolytic allergen., Meno K, Thorsted PB, Ipsen H, Kristensen O, Larsen JN, Spangfort MD, Gajhede M, Lund K, J Immunol. 2005 Sep 15;175(6):3835-45. PMID:16148130

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