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1xmd

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[[Image:1xmd.gif|left|200px]]
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{{Seed}}
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{{STRUCTURE_1xmd| PDB=1xmd | SCENE= }}
{{STRUCTURE_1xmd| PDB=1xmd | SCENE= }}
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'''M335V mutant structure of mouse carnitine octanoyltransferase'''
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===M335V mutant structure of mouse carnitine octanoyltransferase===
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==Overview==
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Carnitine acyltransferases have crucial functions in fatty acid metabolism. Members of this enzyme family show distinctive substrate preferences for short-, medium- or long-chain fatty acids. The molecular mechanism for this substrate selectivity is not clear as so far only the structure of carnitine acetyltransferase has been determined. To further our understanding of these important enzymes, we report here the crystal structures at up to 2.0-A resolution of mouse carnitine octanoyltransferase alone and in complex with the substrate octanoylcarnitine. The structures reveal significant differences in the acyl group binding pocket between carnitine octanoyltransferase and carnitine acetyltransferase. Amino acid substitutions and structural changes produce a larger hydrophobic pocket that binds the octanoyl group in an extended conformation. Mutation of a single residue (Gly-553) in this pocket can change the substrate preference between short- and medium-chain acyl groups. The side chains of Cys-323 and Met-335 at the bottom of this pocket assume dual conformations in the substrate complex, and mutagenesis studies suggest that the Met-335 residue is important for catalysis.
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(as it appears on PubMed at http://www.pubmed.gov), where 15492013 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15492013}}
==About this Structure==
==About this Structure==
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[[Category: Mutant]]
[[Category: Mutant]]
[[Category: Octanoyltransferase]]
[[Category: Octanoyltransferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:12:58 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 06:06:19 2008''

Revision as of 03:06, 28 July 2008

Image:1xmd.png

Template:STRUCTURE 1xmd

M335V mutant structure of mouse carnitine octanoyltransferase

Template:ABSTRACT PUBMED 15492013

About this Structure

1XMD is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Crystal structure of mouse carnitine octanoyltransferase and molecular determinants of substrate selectivity., Jogl G, Hsiao YS, Tong L, J Biol Chem. 2005 Jan 7;280(1):738-44. Epub 2004 Oct 17. PMID:15492013

Page seeded by OCA on Mon Jul 28 06:06:19 2008

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