1xn1

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{{STRUCTURE_1xn1| PDB=1xn1 | SCENE= }}
{{STRUCTURE_1xn1| PDB=1xn1 | SCENE= }}
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'''Crystal Structure Of Lumazine Synthase From Brucella Abortus (Orthorhombic Form At 3.05 Angstroms)'''
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===Crystal Structure Of Lumazine Synthase From Brucella Abortus (Orthorhombic Form At 3.05 Angstroms)===
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==Overview==
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The enzyme lumazine synthase (LS) catalyzes the penultimate step of riboflavin biosynthesis in plants, fungi and bacteria. The quaternary structure of the polypeptide differs between species, existing as pentamers or as icosahedrally arranged dodecamers of pentamers with 60 subunits. The pathogen Brucella spp. expresses two proteins that exhibit LS activity, RibH1 and RibH2. The latter enzyme belongs to a novel third category of quaternary arrangement for LS, that of a decameric structure assembled as a head-to-head oriented dimer of pentamers. In contrast, the RibH1 enzyme is assembled as a pentamer, as noted for several other LS enzymes. RibH1 appears to be the functional LS in Brucella spp., whereas RibH2, an enzyme of lower catalytic activity, is a virulence factor presumably acting in response to oxidative stress. The latter observation prompted us to further investigate the structural and catalytic properties of RibH2 in order to clarify the biological function of this enzyme. Here, we present a detailed analysis of two new crystallographic forms of RibH2 that explain the low catalytic activity of this enzyme in comparison with RibH1 and other LSs. Additionally, we analyze the effect of pH on the structure of this enzyme, and the binding of riboflavin and 6,7-dimethyl-8-ribityllumazine to its active site.
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(as it appears on PubMed at http://www.pubmed.gov), where 16165152 is the PubMed ID number.
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{{ABSTRACT_PUBMED_16165152}}
==About this Structure==
==About this Structure==
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[[Category: Zylberman, V.]]
[[Category: Zylberman, V.]]
[[Category: Transferase]]
[[Category: Transferase]]
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Revision as of 09:40, 27 July 2008

Image:1xn1.png

Template:STRUCTURE 1xn1

Crystal Structure Of Lumazine Synthase From Brucella Abortus (Orthorhombic Form At 3.05 Angstroms)

Template:ABSTRACT PUBMED 16165152

About this Structure

1XN1 is a Single protein structure of sequence from Brucella abortus. Full crystallographic information is available from OCA.

Reference

Crystallographic studies on decameric Brucella spp. Lumazine synthase: a novel quaternary arrangement evolved for a new function?, Klinke S, Zylberman V, Vega DR, Guimaraes BG, Braden BC, Goldbaum FA, J Mol Biol. 2005 Oct 14;353(1):124-37. PMID:16165152

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