1xnc

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1xnc.gif|left|200px]]
+
{{Seed}}
 +
[[Image:1xnc.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1xnc| PDB=1xnc | SCENE= }}
{{STRUCTURE_1xnc| PDB=1xnc | SCENE= }}
-
'''THERMOSTABILIZATION OF THE BACILLUS CIRCULANS XYLANASE, BY THE INTRODUCTION OF DISULFIDE BONDS'''
+
===THERMOSTABILIZATION OF THE BACILLUS CIRCULANS XYLANASE, BY THE INTRODUCTION OF DISULFIDE BONDS===
-
==Overview==
+
<!--
-
The thermostability of the 20 396 Da Bacillus circulans xylanase was increased by the introduction of both intra- and intermolecular disulfide bridges by site-directed mutagenesis. Based on the 3-D structure of the enzyme, sites were chosen where favourable geometry for a bridge existed; in one case, to obtain favourable geometry additional mutations around the cysteine sites were designed by computer modelling. The disulfide bonds introduced into the xylanase were mostly buried and, in the absence of protein denaturants, relatively insensitive to reduction by dithiothreitol. The mutant proteins were examined for residual enzymatic activity after various thermal treatments, and were assayed for enzymatic activity at elevated temperatures to assess their productivity. We have examined one of these mutants by X-ray crystallography. All of the disulfide bond designs tested increased the thermostability of the B. circulans xylanase, but not all enhanced the activity of the enzyme at elevated temperatures.
+
The line below this paragraph, {{ABSTRACT_PUBMED_7700870}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 7700870 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_7700870}}
==About this Structure==
==About this Structure==
Line 25: Line 29:
[[Category: Campbell, R L.]]
[[Category: Campbell, R L.]]
[[Category: Glycosidase]]
[[Category: Glycosidase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:15:34 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 08:34:13 2008''

Revision as of 05:34, 29 July 2008

Image:1xnc.png

Template:STRUCTURE 1xnc

THERMOSTABILIZATION OF THE BACILLUS CIRCULANS XYLANASE, BY THE INTRODUCTION OF DISULFIDE BONDS

Template:ABSTRACT PUBMED 7700870

About this Structure

1XNC is a Single protein structure of sequence from Bacillus circulans. Full crystallographic information is available from OCA.

Reference

Thermostabilization of the Bacillus circulans xylanase by the introduction of disulfide bonds., Wakarchuk WW, Sung WL, Campbell RL, Cunningham A, Watson DC, Yaguchi M, Protein Eng. 1994 Nov;7(11):1379-86. PMID:7700870

Page seeded by OCA on Tue Jul 29 08:34:13 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xnc"
Personal tools