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1xnj

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[[Image:1xnj.gif|left|200px]]
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{{STRUCTURE_1xnj| PDB=1xnj | SCENE= }}
{{STRUCTURE_1xnj| PDB=1xnj | SCENE= }}
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'''APS complex of human PAPS synthetase 1'''
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===APS complex of human PAPS synthetase 1===
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==Overview==
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The high energy sulfate donor 3'-phosphoadenosine-5-phosphosulfate (PAPS) is used for sulfate conjugation of extracellular matrix, hormones and drugs. Human PAPS synthetase 1 catalyzes two subsequent reactions starting from ATP and sulfate. First the ATP sulfurylase domain forms APS, then the APS kinase domain phosphorylates the APS intermediate to PAPS. Up to now the interaction between the two enzymatic activities remained elusive, mainly because of missing structural information. Here we present the crystal structure of human PAPSS1 at 1.8 angstroms resolution. The structure reveals a homodimeric, asymmetric complex with the shape of a chair. The two kinase domains adopt different conformational states, with only one being able to bind its two substrates. The asymmetric binding of ADP to the APS kinase is not only observed in the crystal structure, but can also be detected in solution, using an enzymatic assay. These observations strongly indicate structural changes during the reaction cycle. Furthermore crystals soaked with ADP and APS could be prepared and the corresponding structures could be solved.
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The line below this paragraph, {{ABSTRACT_PUBMED_15755455}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15755455 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15755455}}
==About this Structure==
==About this Structure==
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[[Category: Phosphoadenosine phosphosulfate]]
[[Category: Phosphoadenosine phosphosulfate]]
[[Category: Transferase]]
[[Category: Transferase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:16:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 17:45:08 2008''

Revision as of 14:45, 28 July 2008

Image:1xnj.png

Template:STRUCTURE 1xnj

APS complex of human PAPS synthetase 1

Template:ABSTRACT PUBMED 15755455

About this Structure

1XNJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The crystal structure of human PAPS synthetase 1 reveals asymmetry in substrate binding., Harjes S, Bayer P, Scheidig AJ, J Mol Biol. 2005 Apr 1;347(3):623-35. Epub 2005 Jan 26. PMID:15755455

Page seeded by OCA on Mon Jul 28 17:45:08 2008

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