1xns

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{{STRUCTURE_1xns| PDB=1xns | SCENE= }}
{{STRUCTURE_1xns| PDB=1xns | SCENE= }}
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'''Peptide trapped Holliday junction intermediate in Cre-loxP recombination'''
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===Peptide trapped Holliday junction intermediate in Cre-loxP recombination===
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==Overview==
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Cre recombinase is a prototypical member of the tyrosine recombinase family of site-specific recombinases. Members of this family of enzymes catalyze recombination between specific DNA sequences by cleaving and exchanging one pair of strands between the two substrate sites to form a 4-way Holliday junction (HJ) intermediate and then resolve the HJ intermediate to recombinant products by a second round of strand exchanges. Recently, hexapeptide inhibitors have been described that are capable of blocking the second strand exchange step in the tyrosine recombinase recombination pathway, leading to an accumulation of the HJ intermediate. These peptides are active in the lambda-integrase, Cre recombinase, and Flp recombinase systems and are potentially important tools for both in vitro mechanistic studies and as in vivo probes of cellular function. Here we present biochemical and crystallographic data that support a model where the peptide inhibitor binds in the center of the recombinase-bound DNA junction and interacts with solvent-exposed bases near the junction branch point. Peptide binding induces large conformational changes in the DNA strands of the HJ intermediate, which affect the active site geometries in the recombinase subunits.
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(as it appears on PubMed at http://www.pubmed.gov), where 15591069 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15591069}}
==About this Structure==
==About this Structure==
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[[Category: Peptide inhibitor]]
[[Category: Peptide inhibitor]]
[[Category: Recombination]]
[[Category: Recombination]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:16:27 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 04:10:42 2008''

Revision as of 01:10, 28 July 2008

Image:1xns.png

Template:STRUCTURE 1xns

Peptide trapped Holliday junction intermediate in Cre-loxP recombination

Template:ABSTRACT PUBMED 15591069

About this Structure

1XNS is a Single protein structure of sequence from Enterobacteria phage p1. Full crystallographic information is available from OCA.

Reference

Peptide trapping of the Holliday junction intermediate in Cre-loxP site-specific recombination., Ghosh K, Lau CK, Guo F, Segall AM, Van Duyne GD, J Biol Chem. 2005 Mar 4;280(9):8290-9. Epub 2004 Dec 8. PMID:15591069

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