1xqd

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
-
[[Image:1xqd.jpg|left|200px]]
+
{{Seed}}
 +
[[Image:1xqd.png|left|200px]]
<!--
<!--
Line 9: Line 10:
{{STRUCTURE_1xqd| PDB=1xqd | SCENE= }}
{{STRUCTURE_1xqd| PDB=1xqd | SCENE= }}
-
'''Crystal structure of P450NOR complexed with 3-pyridinealdehyde adenine dinucleotide'''
+
===Crystal structure of P450NOR complexed with 3-pyridinealdehyde adenine dinucleotide===
-
==Overview==
+
<!--
-
Nitric oxide reductase cytochrome P450nor catalyzes an unusual reaction, direct electron transfer from NAD(P)H to bound heme. Here, we succeeded in determining the crystal structure of P450nor in a complex with an NADH analogue, nicotinic acid adenine dinucleotide, which provides conclusive evidence for the mechanism of the unprecedented electron transfer. Comparison of the structure with those of dinucleotide-free forms revealed a global conformational change accompanied by intriguing local movements caused by the binding of the pyridine nucleotide. Arg64 and Arg174 fix the pyrophosphate moiety upon the dinucleotide binding. Stereo-selective hydride transfer from NADH to NO-bound heme was suggested from the structure, the nicotinic acid ring being fixed near the heme by the conserved Thr residue in the I-helix and the upward-shifted propionate side-chain of the heme. A proton channel near the NADH channel is formed upon the dinucleotide binding, which should direct continuous transfer of the hydride and proton. A salt-bridge network (Glu71-Arg64-Asp88) was shown to be crucial for a high catalytic turnover.
+
The line below this paragraph, {{ABSTRACT_PUBMED_15313618}}, adds the Publication Abstract to the page
 +
(as it appears on PubMed at http://www.pubmed.gov), where 15313618 is the PubMed ID number.
 +
-->
 +
{{ABSTRACT_PUBMED_15313618}}
==About this Structure==
==About this Structure==
Line 32: Line 36:
[[Category: Nadh complex]]
[[Category: Nadh complex]]
[[Category: Nitric oxide reductase]]
[[Category: Nitric oxide reductase]]
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:22:06 2008''
+
 
 +
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 11:49:14 2008''

Revision as of 08:49, 29 July 2008

Image:1xqd.png

Template:STRUCTURE 1xqd

Crystal structure of P450NOR complexed with 3-pyridinealdehyde adenine dinucleotide

Template:ABSTRACT PUBMED 15313618

About this Structure

1XQD is a Single protein structure of sequence from Fusarium oxysporum. Full crystallographic information is available from OCA.

Reference

Structural evidence for direct hydride transfer from NADH to cytochrome P450nor., Oshima R, Fushinobu S, Su F, Zhang L, Takaya N, Shoun H, J Mol Biol. 2004 Sep 3;342(1):207-17. PMID:15313618

Page seeded by OCA on Tue Jul 29 11:49:14 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xqd"
Personal tools