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1xty

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{{STRUCTURE_1xty| PDB=1xty | SCENE= }}
{{STRUCTURE_1xty| PDB=1xty | SCENE= }}
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'''Crystal structure of Sulfolobus solfataricus peptidyl-tRNA hydrolase'''
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===Crystal structure of Sulfolobus solfataricus peptidyl-tRNA hydrolase===
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==Overview==
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The 3-D structure of the peptidyl-tRNA hydrolase from the archaea Sulfolobus solfataricus has been solved at 1.8 A resolution. Homologues of this enzyme are found in archaea and eucarya. Bacteria display a different type of peptidyl-tRNA hydrolase that is also encountered in eucarya. In solution, the S. solfataricus hydrolase behaves as a dimer. In agreement, the crystalline structure of this enzyme indicates the formation of a dimer. Each protomer is made of a mixed five-stranded beta-sheet surrounded by two groups of two alpha-helices. The dimer interface is mainly formed by van der Waals interactions between hydrophobic residues belonging to the two N-terminal alpha1 helices contributed by two protomers. Site-directed mutagenesis experiments were designed for probing the basis of specificity of the archaeal hydrolase. Among the strictly conserved residues within the archaeal/eucaryal peptidyl-tRNA hydrolase family, three residues, K18, D86, and T90, appear of utmost importance for activity. They are located in the N-part of alpha1 and in the beta3-beta4 loop. K18 and D86, which form a salt bridge, might play a role in the catalysis thanks to their acid and basic functions, whereas the OH group of T90 could act as a nucleophile. These observations clearly distinguish the active site of the archaeal/eucaryal hydrolases from that of the bacterial/eucaryal ones, where a histidine is believed to serve as the catalytic base.
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(as it appears on PubMed at http://www.pubmed.gov), where 15766258 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15766258}}
==About this Structure==
==About this Structure==
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[[Category: Schmitt, E.]]
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[[Category: Mixed beta sheet]]
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Revision as of 13:40, 29 July 2008

Image:1xty.png

Template:STRUCTURE 1xty

Crystal structure of Sulfolobus solfataricus peptidyl-tRNA hydrolase

Template:ABSTRACT PUBMED 15766258

About this Structure

1XTY is a Single protein structure of sequence from Pyrococcus abyssi. Full crystallographic information is available from OCA.

Reference

Crystal structure at 1.8 A resolution and identification of active site residues of Sulfolobus solfataricus peptidyl-tRNA hydrolase., Fromant M, Schmitt E, Mechulam Y, Lazennec C, Plateau P, Blanquet S, Biochemistry. 2005 Mar 22;44(11):4294-301. PMID:15766258

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