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| | {{STRUCTURE_1xue| PDB=1xue | SCENE= }} | | {{STRUCTURE_1xue| PDB=1xue | SCENE= }} |
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| - | '''STRUCTURE OF GTGGAATGCAATGGAAC HAIRPIN, NMR, 10 STRUCTURES'''
| + | ===STRUCTURE OF GTGGAATGCAATGGAAC HAIRPIN, NMR, 10 STRUCTURES=== |
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| - | ==Overview==
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| - | Recently, we established that satellite III (TGGAA)n tandem repeats, which occur at the centromeres of human chromosomes, pair with themselves to form an unusual "self-complementary" antiparallel duplex containing (GGA)2 motifs in which two unpaired guanines from opposite strands intercalate between sheared G.A base pairs. In separate studies, we have also established that the GCA triplet does not form bimolecular (GCA)2 motifs but instead promotes the formation of hairpins containing a GCA-turn motif in which the loop contains a single cytidine closed by a sheared G.A pair. Since TGCAA is the most frequent variant of TGGAA found in satellite III repeats, we reasoned that the potential of this variant to form GCA-turn miniloop fold-back structures might be an important factor in modulating the local structure in natural (TGGAA)n repeats. We report here the NMR-derived solution structure of the heptadecadeoxynucleotide (G)TGGAATGCAATGGAA(C) in which a central TGCAA pentamer is flanked by two TGGAA pentamers. This 17-mer forms a rather unusual and very stable hairpin structure containing eight base pairs in the stem, only four of which are Watson-Crick pairs, and a loop consisting of a single cytidine residue. The stem contains a (GGA)2 motif with intercalative 14G/4G stacking between two sheared G.A base pairs; the loop end of the stem consists of a sheared 8G.10A closing pair with the cytosine base of the 9C loop stacked on 8G. The remarkable stability of this unusual hairpin structure (Tm = 63 degrees C) suggests that it probably plays an important role in modulating the folding of satellite III (TGGAA)n repeats at the centromere.
| + | The line below this paragraph, {{ABSTRACT_PUBMED_8901550}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 8901550 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_8901550}} |
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| | ==About this Structure== | | ==About this Structure== |
| - | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XUE OCA]. | + | Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XUE OCA]. |
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| | ==Reference== | | ==Reference== |
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| | [[Category: Mutation]] | | [[Category: Mutation]] |
| | [[Category: Satellite iii]] | | [[Category: Satellite iii]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:31:24 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 11:25:47 2008'' |
Revision as of 08:25, 28 July 2008
Template:STRUCTURE 1xue
STRUCTURE OF GTGGAATGCAATGGAAC HAIRPIN, NMR, 10 STRUCTURES
Template:ABSTRACT PUBMED 8901550
About this Structure
Full experimental information is available from OCA.
Reference
A single G-to-C change causes human centromere TGGAA repeats to fold back into hairpins., Zhu L, Chou SH, Reid BR, Proc Natl Acad Sci U S A. 1996 Oct 29;93(22):12159-64. PMID:8901550
Page seeded by OCA on Mon Jul 28 11:25:47 2008
