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| - | [[Image:1xwr.gif|left|200px]] | + | {{Seed}} |
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| | {{STRUCTURE_1xwr| PDB=1xwr | SCENE= }} | | {{STRUCTURE_1xwr| PDB=1xwr | SCENE= }} |
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| - | '''Crystal structure of the coliphage lambda transcription activator protein CII'''
| + | ===Crystal structure of the coliphage lambda transcription activator protein CII=== |
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| - | ==Overview==
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| - | The temperate coliphage lambda, after infecting its host bacterium Escherichia coli, can develop either along the lytic or the lysogenic pathway. Crucial to the lysis/lysogeny decision is the homotetrameric transcription-activator protein CII (4 x 11 kDa) of the phage that binds to a unique direct-repeat sequence T-T-G-C-N6-T-T-G-C at each of the three phage promoters it activates: p(E), p(I), and p(aQ). Several regions of CII have been identified for its various functions (DNA binding, oligomerization, and susceptibility to host protease), but the crystal structure of the protein long remained elusive. Here, we present the three-dimensional structure of CII at 2.6-angstroms resolution. The CII monomer is comprised of four alpha helices and a disordered C terminus. The first three helices (alpha1-alpha3) form a compact domain, whereas the fourth helix (alpha4) protrudes in different orientations in each subunit. A four-helix bundle, formed by alpha4 from each subunit, holds the tetramer. The quaternary structure can be described as a dimer of dimers, but the tetramer does not exhibit a closed symmetry. This unusual quaternary arrangement allows the placement of the helix-turn-helix motifs of two of the four CII subunits for interaction with successive major grooves of B-DNA, from one face of DNA. This structure provides a simple explanation for how a homotetrameric protein may recognize a direct-repeat DNA sequence rather than the inverted-repeat sequences of most prokaryotic activators. | + | The line below this paragraph, {{ABSTRACT_PUBMED_16061804}}, adds the Publication Abstract to the page |
| | + | (as it appears on PubMed at http://www.pubmed.gov), where 16061804 is the PubMed ID number. |
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| | + | {{ABSTRACT_PUBMED_16061804}} |
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| | ==About this Structure== | | ==About this Structure== |
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| | [[Category: All-alpha fold]] | | [[Category: All-alpha fold]] |
| | [[Category: Dna-binding protein]] | | [[Category: Dna-binding protein]] |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:36:16 2008'' | + | |
| | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Tue Jul 29 14:43:03 2008'' |
Revision as of 11:43, 29 July 2008
Template:STRUCTURE 1xwr
Crystal structure of the coliphage lambda transcription activator protein CII
Template:ABSTRACT PUBMED 16061804
About this Structure
1XWR is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.
Reference
Structure of lambda CII: implications for recognition of direct-repeat DNA by an unusual tetrameric organization., Datta AB, Panjikar S, Weiss MS, Chakrabarti P, Parrack P, Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11242-7. Epub 2005 Aug 1. PMID:16061804
Page seeded by OCA on Tue Jul 29 14:43:03 2008
