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1y4h

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[[Image:1y4h.gif|left|200px]]
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{{Seed}}
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[[Image:1y4h.png|left|200px]]
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{{STRUCTURE_1y4h| PDB=1y4h | SCENE= }}
{{STRUCTURE_1y4h| PDB=1y4h | SCENE= }}
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'''Wild type staphopain-staphostatin complex'''
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===Wild type staphopain-staphostatin complex===
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==Overview==
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Staphostatins are the endogenous, highly specific inhibitors of staphopains, the major secreted cysteine proteases from Staphylococcus aureus. We have previously shown that staphostatins A and B are competitive, active site-directed inhibitors that span the active site clefts of their target proteases in the same orientation as substrates. We now report the crystal structure of staphostatin B in complex with wild-type staphopain B at 1.9 A resolution. In the complex structure, the catalytic residues are found in exactly the positions that would be expected for uncomplexed papain-type proteases. There is robust, continuous density for the staphostatin B binding loop and no indication for cleavage of the peptide bond that comes closest to the active site cysteine of staphopain B. The carbonyl carbon atom C of this peptide bond is 4.1 A away from the active site cysteine sulfur Sgamma atom. The carbonyl oxygen atom O of this peptide bond points away from the putative oxyanion hole and lies almost on a line from the Sgamma atom to the C atom. The arrangement is strikingly similar to the "ionmolecule" arrangement for the complex of papain-type enzymes with their substrates but differs significantly from the arrangement conventionally assumed for the Michaelis complex of papain-type enzymes with their substrates and also from the arrangement that is crystallographically observed for complexes of standard mechanism inhibitors and their target serine proteases.
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The line below this paragraph, {{ABSTRACT_PUBMED_15644332}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 15644332 is the PubMed ID number.
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{{ABSTRACT_PUBMED_15644332}}
==About this Structure==
==About this Structure==
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[[Category: Staphopain b]]
[[Category: Staphopain b]]
[[Category: Staphostatin b]]
[[Category: Staphostatin b]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 15:52:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Jul 28 19:12:24 2008''

Revision as of 16:12, 28 July 2008

Image:1y4h.png

Template:STRUCTURE 1y4h

Wild type staphopain-staphostatin complex

Template:ABSTRACT PUBMED 15644332

About this Structure

1Y4H is a Protein complex structure of sequences from Staphylococcus aureus. Full crystallographic information is available from OCA.

Reference

A comparison of staphostatin B with standard mechanism serine protease inhibitors., Filipek R, Potempa J, Bochtler M, J Biol Chem. 2005 Apr 15;280(15):14669-74. Epub 2005 Jan 11. PMID:15644332

Page seeded by OCA on Mon Jul 28 19:12:24 2008

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